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Volume 23, Number 6—June 2017

Research

Distribution and Quantitative Estimates of Variant Creutzfeldt-Jakob Disease Prions in Tissues of Clinical and Asymptomatic Patients

Jean Y. Douet, Caroline Lacroux, Naima Aron, Mark W. Head, Séverine Lugan, Cécile Tillier, Alvina Huor, Hervé Cassard, Mark Arnold, Vincent Beringue, James W. Ironside, and Olivier AndréolettiComments to Author 
Author affiliations: Institut National de la Recherche Agronomique, Toulouse, France (J.Y. Douet, C. Lacroux, N. Aron, S. Lugan, C. Tillier, A. Huor, H. Cassard, O. Andréoletti); University of Edinburgh, Edinburgh, Scotland, UK (M.W. Head, J.W. Ironside); Animal and Plant Health Agency, Loughborough, UK (M. Arnold); Institut National de la Recherche Agronomique, Jouy-en-Josas, France (V. Beringue)

Main Article

Table 1

Endpoint titration of reference sample from a patient with vCJD in tgBov mice expressing bovine prion protein*

Dilution Transmission in tgBov mice
No. positive mice/no. tested Mean ± SD, incubation, d
Undiluted 6/6 249 ± 2
10−1 6/6 283 ± 15
10−2 6/6 316 ± 21
10−3 6/6 342 ± 10
10−4 6/6 453 ± 66
10−5 2/6 479, 495†
10−6 1/6 502†
10−7 0/6 >700

*A 10% (wt/vol) homogenate was prepared by using frontal cortex from a clinically affected patient with vCJD. Groups of 6 tgBov mice were inoculated intracerebrally with 20 μL of serial 10-fold dilutions of this homogenate. Mice were considered positive when abnormal prion protein deposition was detected in the brain. vCJD, variant Creutzfeldt-Jakob disease.
†Dilutions at which <50% of mice were positive.

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