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Volume 23, Number 8—August 2017
Synopsis

Added Value of Next-Generation Sequencing for Multilocus Sequence Typing Analysis of a Pneumocystis jirovecii Pneumonia Outbreak1

Elena Charpentier, Cécile Garnaud, Claire Wintenberger, Sébastien Bailly, Jean-Benjamin Murat2, John Rendu, Patricia Pavese, Thibault Drouet, Caroline Augier, Paolo Malvezzi, Anne Thiébaut-Bertrand, Marie-Reine Mallaret, Olivier Epaulard, Muriel Cornet, Sylvie Larrat, and Danièle MaubonComments to Author 
Author affiliations: Centre Hospitalier Universitaire Grenoble Alpes, Grenoble, France (E. Charpentier, C. Garnaud, C. Wintenberger, J.-B. Murat, J. Rendu, P. Pavese, T. Drouet, C. Augier, P. Malvezzi, A. Thiébaut-Bertrand, M.-R. Mallaret, O. Epaulard, M. Cornet, S. Larrat, D. Maubon); Université Grenoble Alpes, Grenoble (E. Charpentier, C. Garnaud, M.-R. Mallaret, O. Epaulard, M. Cornet, S. Larrat, D. Maubon); Institut National de la Santé et de la Recherche Médicale Université Paris Diderot, Paris, France (S. Bailly).

Main Article

Table 1

Main clinical and biologic characteristics of the 12 patients who had received solid organ transplants and 20 control patients in a study of a Pneumocystis jirovecii pneumonia outbreak at a university hospital in France, 2009–2015*

Patient Age, y/sex Date of diagnosis Underlying condition Time since transplant, mo Sample type Fungal load, MSG copies/mL Outcome
T1 34/F 2014 May 20  Pulmonary transplant 60 BAL 1.1 × 109 Died
T2 59/F 2014 May 27 Heart and kidney transplant 300 (H) – 2 (K) BAL 3.9 × 108 Survived
T3 57/F 2014 Jun 19 Heart transplant 137 BAL 2.7 × 108 Died
T4 65/M 2014 Jul 7 Heart transplant 37 BAL 1.5 × 108 Died
T5 66/M 2014 Aug 19 Kidney transplant 4 Sputum 6.6 × 1010 Survived
T6 48/M 2014 Aug 29 Heart transplant 78 BAL 1 × 106 Survived
T7 74/F 2014 Oct 29 Kidney transplant 128 BAL 2 × 107 Died
T8 69/F 2014 Nov 24 Kidney transplant 23 BAL 8.8 × 106 Survived
T9 63/M 2015 Jan 7 Liver transplant 5 BAL 1.1 × 104 Survived
T10 69/M 2015 Jan 10 Kidney transplant 228 Sputum 1.2 × 105 Survived
T11 61/F 2015 Mar 3 Heart transplant 122 BAL 3.1 × 103 Survived
T12 75/F 2015 Aug 26 Heart transplant 95 BAL 2 × 105 Survived
C1 56/M 2012 Aug 25 Liver transplant 6 BA 6 × 106 Died (HCV relapse)
C2 52/M 2013 May 17 Pulmonary transplant 21 BAL 1.7 × 105 Survived
C3 82/F 2012 Mar 9 Rheumatoid polyarthritis – BAL 5.6 × 103 Survived
C4 62/M 2013 Aug 29 NHL – BAL 4.3 × 108 Survived
C5 66/M 2013 Sep 10 NHL – BAL 1.2 × 106 Survived
C6 25/M 2014 Feb 27 HIV – BAL 4.2 × 108 Survived
C7 1/F 2014 Apr 9 Primary immunodeficiency – BAL 5.2 × 109 Survived
C8 56/M 2014 Apr 19 HIV – BAL 4.3 × 109 Survived
C9 47/F 2014 Apr 24 Allografted AML with GVHD – BAL 1.4 × 106 Survived
C10 74/M 2014 Jul 1 NHL – BAL 6.1 × 108 Survived
C11 77/M 2014 Sep 4 MPN and NHL – BAL 1.2 × 105 Survived
C12 82/M 2014 Sep 5 NHL – BAL 5.8 × 104 Survived
C13 68/M 2014 Oct 10 Glioblastoma – BAL 3.4 × 1010 Survived
C14 7 mo/F 2014 Oct 15 Primary immunodeficiency – Sputum 4.7 × 106 Survived
C15 75/F 2014 Oct 23 B-chronic lymphoid leukemia – BAL 3.4 × 105 Survived
C16 82/F 2014 Dec 5 B-chronic lymphoid leukemia – BAL 1.1 × 104 Survived
C17 32/F 2015 Feb 27 Hodgkin's disease – BAL 1.2 × 104 Survived
C18 50/M 2015 Feb 28 Hodgkin's disease – BAL 6.4 × 103 Survived
C19 78/M 2015 Mar 18 B-chronic lymphoid leukemia – BAL 5.9 × 106 Survived
C20
 75/M
 2015 Mar 22
 NHL
 –
 BA
 3.9 × 104
 Died
*T1–T12 indicate the 12 solid organ transplant patients from the cluster; C1–C20 indicate the 20 control patients. AML, acute myeloid leukemia; BA, bronchoaspiration; BAL, bronchoalveolar lavage fluids; GVHD, graft-versus-host disease; H, heart; K, kidney; MPN, myeloproliferative neoplasm; MSG, major surface glycoprotein; NA, not applicable; ND, not done; NHL, non-Hodgkin lymphoma; –, nonexistent or unspecified.

Main Article

1Preliminary results from this study were presented at the American Society for Microbiology Microbe 2016 Conference, June 16–20, 2016, Boston, Massachusetts, USA.

2Current affiliation: Centre Hospitalier Universitaire Limoges, Limoges, France.

Page created: July 17, 2017
Page updated: July 17, 2017
Page reviewed: July 17, 2017
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