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Volume 24, Number 1—January 2018
Research

Detection and Circulation of a Novel Rabbit Hemorrhagic Disease Virus in Australia

Jackie E. Mahar, Andrew J. Read, Xingnian Gu, Nadya Urakova1, Roslyn Mourant, Melissa Piper, Stéphanie Haboury, Edward C. Holmes, Tanja Strive, and Robyn N. HallComments to Author 
Author affiliations: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia (J.E. Mahar, N. Urakova, R. Mourant, M. Piper, S. Haboury, T. Strive, R.N. Hall); The University of Sydney, Sydney, New South Wales, Australia (J.E. Mahar, E.C. Holmes); Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia (A.J. Read, X. Gu); Invasive Animals Cooperative Research Centre, Bruce, Australian Capital Territory, Australia (N. Urakova, T. Strive, R.N. Hall)

Main Article

Figure 1

Genomic organization of RHDV detected in rabbits, Australia. A) RHDV has a polyadenylated single-stranded positive-sense gRNA of ≈7.4 kb consisting of 2 ORFs (open boxes), with the VP (open circle) covalently attached to the 5′ end. ORF1 encodes a polyprotein that is proteolytically cleaved to produce the major capsid protein, VP60, and the nonstructural proteins p16, p23, 2C-like protein (2C-like), p29, VPg, the viral protease, and the RdRp. ORF2 encodes the minor structural protein, VP10. B) A

Figure 1. Genomic organization of RHDV. Top panel: RHDV has a polyadenylated single-stranded positive-sense gRNA of ≈7.4 kb consisting of 2 ORFs (open boxes), with the VPg (open circle) covalently attached to the 5′ end. ORF1 encodes a polyprotein that is proteolytically cleaved to produce the major capsid protein, VP60, and the nonstructural proteins p16, p23, 2C-like protein (2C-like), p29, VPg, the viral protease, and the RdRp. ORF2 encodes the minor structural protein, VP10. Bottom panel: A 2.1-kb, VPg-linked, polyadenylated sgRNA is collinear with the 3′ end of the gRNA and encodes VP60 and VP10. High homology between the 5′ end of the sgRNA and the corresponding region of the gRNA (the RdRp and capsid junction) may facilitate template switching during replication. gRNA, RNA genome; ORF, open reading frame; Pro, protease; RHDV, rabbit hemorrhagic disease virus; RdRp, RNA-dependent RNA polymerase; sgRNA, subgenomic RNA; VP, viral protein; VPg, viral genome-linked protein.

Main Article

1Current affiliation: University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA.

Page created: January 09, 2018
Page updated: January 09, 2018
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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