Table 3

Detection and Circulation of a Novel Rabbit Hemorrhagic Disease Virus in Australia

Jackie E. Mahar, Andrew J. Read, Xingnian Gu, Nadya Urakova¹, Roslyn Mourant, Melissa Piper, Stéphanie Haboury, Edward C. Holmes, Tanja Strive, and Robyn N. Hall

Author affiliations: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia (J.E. Mahar, N. Urakova, R. Mourant, M. Piper, S. Haboury, T. Strive, R.N. Hall); The University of Sydney, Sydney, New South Wales, Australia (J.E. Mahar, E.C. Holmes); Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia (A.J. Read, X. Gu); Invasive Animals Cooperative Research Centre, Bruce, Australian Capital Territory, Australia (N. Urakova, T. Strive, R.N. Hall)

Main Article

Cross-reactivity of different diagnostic tests used to identify novel RHDVa variant in rabbits, Australia*

Test	RHDV	RHDVa	RCV-A1
RHDV antigen capture ELISA	+++	–/+	–
RCV-A1 blocking ELISA	–	–	+++
RHDV competition ELISA	+++	++	–/+
RHDV IgA ELISA	+++	+++	++
RHDV IgM ELISA	+++	+++	++
VP60 rRT-PCR	+++	+++	+++
RHDVXa-2010 rRT-PCR	–	+++	–
Lagovirus RT-PCR	+++	+++	+++

*All tests used the VP60 capsid protein coding region as target region. RCV, rabbit calicivirus; RHDV, rabbit hemorrhagic disease virus; rRT-PCR, real-time reverse transcription PCR; VP, viral protein; –, no cross-reactivity; –/+, minimal cross-reactivity; ++, moderate cross-reactivity; +++, marked cross-reactivity.

Main Article

¹Current affiliation: University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA.

Page created: January 09, 2018

Page updated: January 09, 2018

Page reviewed: January 09, 2018

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