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Volume 24, Number 1—January 2018
Research

Detection and Circulation of a Novel Rabbit Hemorrhagic Disease Virus in Australia

Jackie E. Mahar, Andrew J. Read, Xingnian Gu, Nadya Urakova1, Roslyn Mourant, Melissa Piper, Stéphanie Haboury, Edward C. Holmes, Tanja Strive, and Robyn N. HallComments to Author 
Author affiliations: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia (J.E. Mahar, N. Urakova, R. Mourant, M. Piper, S. Haboury, T. Strive, R.N. Hall); The University of Sydney, Sydney, New South Wales, Australia (J.E. Mahar, E.C. Holmes); Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia (A.J. Read, X. Gu); Invasive Animals Cooperative Research Centre, Bruce, Australian Capital Territory, Australia (N. Urakova, T. Strive, R.N. Hall)

Main Article

Figure 3

RDP plot (42) demonstrating recombination in a representative RHDVa strain from Australia. The pairwise identity of the recombinant, KYO-1, with the putative parental strains, RHDVa/AB300693.2/JPN/Hokkaido/2002 (black) and RCV-A1/EU871528.1/AUS/MIC-07(1–4)/2007 (dark gray), is plotted according to genome position (nt). A clear crossover event can be observed at the junction of RNA-dependent RNA polymerase and viral protein 60. The window size was set to 30. A schematic representation of the rabb

Figure 3. Recombination detection program plot (42) demonstrating recombination in a representative rabbit hemorraghic disease virus type a (RHDVa) strain from Australia. The pairwise identity of the recombinant, KYO-1, with the putative parental strains, RHDVa/AB300693.2/JPN/Hokkaido/2002 (black) and RCV-A1/EU871528.1/AUS/MIC-07(1–4)/2007 (dark gray), is plotted according to genome position (nt). A clear crossover event can be observed at the junction of RNA-dependent RNA polymerase and viral protein 60. The window size was set to 30. A schematic representation of the rabbit lagovirus gRNA and sgRNA is shown above the RDP plot to illustrate the genomic structure. The light gray bar shows the region where recombination was detected. gRNA, RNA genome; RHDV, RCV, rabbit calicivirus; sgRNA, subgenomic RNA.

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1Current affiliation: University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA.

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