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Volume 24, Number 1—January 2018
Research

Detection and Circulation of a Novel Rabbit Hemorrhagic Disease Virus in Australia

Jackie E. Mahar, Andrew J. Read, Xingnian Gu, Nadya Urakova1, Roslyn Mourant, Melissa Piper, Stéphanie Haboury, Edward C. Holmes, Tanja Strive, and Robyn N. HallComments to Author 
Author affiliations: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia (J.E. Mahar, N. Urakova, R. Mourant, M. Piper, S. Haboury, T. Strive, R.N. Hall); The University of Sydney, Sydney, New South Wales, Australia (J.E. Mahar, E.C. Holmes); Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia (A.J. Read, X. Gu); Invasive Animals Cooperative Research Centre, Bruce, Australian Capital Territory, Australia (N. Urakova, T. Strive, R.N. Hall)

Main Article

Table 1

Primers and probes used for RHDV and RHDVa detection by real-time reverse transcription PCR in isolates from rabbits, Australia*

Name Sense Sequence, 5′ → 3′ Strain Reference
vp60–7_FOR + ACY TGA CTG AAC TYA TTG ACG RHDV
 (34)
vp60–8_REV – TCA GAC ATA AGA AAA GCC ATT GG (34)
vp60–9_FAM
 Probe
 CCA ARA GCA CRC TCG TGT TCA ACC T–FAM-BHQ1
 Modified from (34)
RHDVXa2010-F1 + GCACCCGGCAGTATTCTC RHDVa This study
RHDVXa2010-R1 – CCCAGCCAGCGTACATCTG This study
RHDVXa2010-P1 Probe ACTGTCCAACACTCTCCACAGAACA–FAM-BHQ1 This study

*RHDV, rabbit hemorrhagic disease virus; vp, viral protein; +, positive; –, negative.

Main Article

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Main Article

1Current affiliation: University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA.

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