Rat Hepatitis E Virus as Cause of Persistent Hepatitis after Liver Transplant
Siddharth Sridhar, Cyril C.Y. Yip, Shusheng Wu, Jianpiao Cai, Anna Jin-Xia Zhang, Kit-Hang Leung, Tom W.H. Chung, Jasper F.W. Chan, Wan-Mui Chan, Jade L.L. Teng, Rex K.H. Au-Yeung, Vincent C.C. Cheng, Honglin Chen, Susanna K.P. Lau, Patrick C.Y. Woo, Ning-Shao Xia, Chung-Mau Lo, and Kwok-Yung Yuen
Author affiliations: The University of Hong Kong, Hong Kong, China (S. Sridhar, C.C.Y. Yip, S. Wu, J. Cai, A.J.-X. Zhang, K.-H. Leung, T.W.H. Chung, J.F.W. Chan, W.-M. Chan, J.L.L. Teng, R.K.H. Au-Yeung, V.C.C. Cheng, H. Chen, S.K.P. Lau, P.C.Y. Woo, C.-M. Lo, K.-Y. Yuen); The University of Hong Kong–Shenzhen Hospital, Shenzhen, China (J.F.W. Chan, C.-M. Lo, K.-Y. Yuen); Xiamen University, Xiamen, China (N.-S. Xia)
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Figure 5
Figure 5. Isolation of HEV-C from 56-year-old male patient’s feces in cell culture, Queen Mary Hospital, Hong Kong. A) HEV-C RNA loads in culture S and day-7 CL of A549, Huh-7, and Caco-2 cell lines after inoculation by patient’s filtered fecal suspension. Mean of 3 replicates; error bars indicate SEM. B) Uninfected A549 cell monolayer stained with anti–HEV-C polyclonal antiserum. C) Infected A549 cell monolayer stained with anti–HEV-C polyclonal antiserum. Original magnification ×400. CL, cell lysate; HEV, hepatitis E virus; S, supernatant.
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