Table 3

Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats

Won-Keun Kim¹, Jin Sun No¹, Seung-Ho Lee, Dong Hyun Song, Daesang Lee, Jeong-Ah Kim, Se Hun Gu, Sunhye Park, Seong Tae Jeong, Heung-Chul Kim, Terry A. Klein, Michael R. Wiley, Gustavo Palacios, and Jin-Won Song

Author affiliations: Korea University, Seoul, South Korea (W.-K. Kim, J.S. No, S.-H. Lee, J.-A. Kim, J.-W. Song); Agency for Defense Development, Daejeon, South Korea (D.H. Song, D. Lee, S.H. Gu, S. Park, S.T. Jeong); 65th Medical Brigade/Medical Department Activity−Korea, Seoul (H.-C. Kim, T.A. Klein); US Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA (M.R. Wiley, G. Palacios)

Main Article

Quantitation and multiplex PCR–based NGS coverages of SEOV RNA, South Korea, 2000–2016*

Species and sample			SEOV genomes, % coverage‡
Species and sample	Origin	C_t†	L segment, nt 1–6530	M segment, nt 1–3651	S segment, nt 1–1769
Human
SEOV Hu02-112	Serum	36.0	85.0	93.2	86.0
SEOV Hu02-180	Serum	28.0	86.2	93.2	87.9
SEOV Hu02-258	Serum	27.5	99.6	99.7	91.6
SEOV Hu02-294	Serum	36.5	68.2	94.5	97.7
SEOV Hu02-529	Serum	32.1	94.9	93.8	97.7
SEOV Hu02-668	Serum	36.8	72.7	93.4	85.1
Rattus norvegicus rats
SEOV Rn10-134§	Lung	27.3	99.6	99.2	98.9
SEOV Rn10-145§	Lung	16.1	99.7	99.7	99.4
SEOV Rn11-44§	Lung	21.3	99.1	99.5	98.3
SEOV Rn11-53§	Lung	27.6	99.6	99.7	98.8

*C_t, cycle threshold; Hu, human; L, large; M, medium; NGS, next-generation sequencing; Rn, rat; S, small; SEOV, Seoul virus.
†Determined by real-time quantitative PCR specific for SEOV S segment.
‡Genome coverages were calculated by obtained consensus sequence matching to genome positions of SEOV 80-39 strain (GenBank accession nos. NC_005238, NC_005237, NC_005236).
§Whole-genome sequences of SEOV L, M, and S segments were obtained by 3′ and 5′ rapid amplification of cDNA ends PCR.

Main Article

¹These authors contributed equally to this article.

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Page updated: January 17, 2018

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