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Volume 24, Number 2—February 2018

Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats

Won-Keun Kim1, Jin Sun No1, Seung-Ho Lee, Dong Hyun Song, Daesang Lee, Jeong-Ah Kim, Se Hun Gu, Sunhye Park, Seong Tae Jeong, Heung-Chul Kim, Terry A. Klein, Michael R. Wiley, Gustavo Palacios, and Jin-Won SongComments to Author 
Author affiliations: Korea University, Seoul, South Korea (W.-K. Kim, J.S. No, S.-H. Lee, J.-A. Kim, J.-W. Song); Agency for Defense Development, Daejeon, South Korea (D.H. Song, D. Lee, S.H. Gu, S. Park, S.T. Jeong); 65th Medical Brigade/Medical Department Activity−Korea, Seoul (H.-C. Kim, T.A. Klein); US Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA (M.R. Wiley, G. Palacios)

Main Article

Table 3

Quantitation and multiplex PCR–based NGS coverages of SEOV RNA, South Korea, 2000–2016*

Species and sample

SEOV genomes, % coverage‡
Origin Ct L segment, nt 1–6530 M segment, nt 1–3651 S segment, nt 1–1769
SEOV Hu02-112 Serum 36.0 85.0 93.2 86.0
SEOV Hu02-180 Serum 28.0 86.2 93.2 87.9
SEOV Hu02-258 Serum 27.5 99.6 99.7 91.6
SEOV Hu02-294 Serum 36.5 68.2 94.5 97.7
SEOV Hu02-529 Serum 32.1 94.9 93.8 97.7
SEOV Hu02-668 Serum 36.8 72.7 93.4 85.1
Rattus norvegicus rats
SEOV Rn10-134§ Lung 27.3 99.6 99.2 98.9
SEOV Rn10-145§ Lung 16.1 99.7 99.7 99.4
SEOV Rn11-44§ Lung 21.3 99.1 99.5 98.3
SEOV Rn11-53§ Lung 27.6 99.6 99.7 98.8

*Ct, cycle threshold; Hu, human; L, large; M, medium; NGS, next-generation sequencing; Rn, rat; S, small; SEOV, Seoul virus.
†Determined by real-time quantitative PCR specific for SEOV S segment.
‡Genome coverages were calculated by obtained consensus sequence matching to genome positions of SEOV 80-39 strain (GenBank accession nos. NC_005238, NC_005237, NC_005236).
§Whole-genome sequences of SEOV L, M, and S segments were obtained by 3′ and 5′ rapid amplification of cDNA ends PCR.

Main Article

1These authors contributed equally to this article.

Page created: January 17, 2018
Page updated: January 17, 2018
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