Volume 24, Number 2—February 2018
Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats
|Species and sample||SEOV genomes, % coverage‡
|Origin||Ct†||L segment, nt 1–6530||M segment, nt 1–3651||S segment, nt 1–1769|
|Rattus norvegicus rats|
*Ct, cycle threshold; Hu, human; L, large; M, medium; NGS, next-generation sequencing; Rn, rat; S, small; SEOV, Seoul virus.
†Determined by real-time quantitative PCR specific for SEOV S segment.
‡Genome coverages were calculated by obtained consensus sequence matching to genome positions of SEOV 80-39 strain (GenBank accession nos. NC_005238, NC_005237, NC_005236).
§Whole-genome sequences of SEOV L, M, and S segments were obtained by 3′ and 5′ rapid amplification of cDNA ends PCR.
1These authors contributed equally to this article.