Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 24, Number 5—May 2018
Dispatch

Equine Encephalosis Virus in India, 2008

Pragya D. Yadav, César G. Albariño, Dimpal A. Nyayanit, Lisa Guerrero, M. Harley Jenks, Prasad Sarkale, Stuart T. Nichol, and Devendra T. MouryaComments to Author 
Author affiliations: National Institute of Virology, Pune, India (P.D. Yadav, D.A. Nyayanit, P. Sarkale, D.T. Mourya); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C.G. Albariño, L. Guerrero, M.H. Jenks, S.T. Nichol)

Main Article

Figure 1

Sample identification by use of next-generation sequencing. RNA reads from blood (A) and lung (B) samples from horse that died of equine encephalosis in India, 2008. Samples were categorized by using Taxonomer software (11); 0.5% of the reads from blood samples (total reads 2,610,292, average length 137 bp) and 12.5% of the reads from lung samples (total reads 2,125,678, average length 135 bp) were matched to those of reoviruses.

Figure 1. Sample identification by use of next-generation sequencing. RNA reads from blood (A) and lung (B) samples from horse that died of equine encephalosis in India, 2008. Samples were categorized by using Taxonomer software (11); 0.5% of the reads from blood samples (total reads 2,610,292, average length 137 bp) and 12.5% of the reads from lung samples (total reads 2,125,678, average length 135 bp) were matched to those of reoviruses.

Main Article

References
  1. Dhama  K, Pawaiya  RVS, Karthik  K, Chakraborty  S, Tiwari  R, Verma  AK. Equine encephalosis virus (EEV): a review. Asian J Anim Vet Adv. 2014;9:123–33. DOIGoogle Scholar
  2. McCall  CA. Control of common infectious horse diseases [cited 2018 Feb 26]. http://www.aces.edu/pubs/docs/A/ANR-0589/index2.tmpl
  3. Westcott  D, Mildenberg  Z, Bellaiche  M, McGowan  SL, Grierson  SS, Choudhury  B, et al. Evidence for the circulation of equine encephalosis virus in Israel since 2001. PLoS One. 2013;8:e70532. DOIPubMedGoogle Scholar
  4. Tirosh-Levy  S, Gelman  B, Zivotofsky  D, Quraan  L, Khinich  E, Nasereddin  A, et al. Seroprevalence and risk factor analysis for exposure to equine encephalosis virus in Israel, Palestine and Jordan. Vet Med Sci. 2017;3:82–90. DOIPubMedGoogle Scholar
  5. Rathogwa  NM, Quan  M, Smit  JQ, Lourens  C, Guthrie  AJ, van Vuuren  M. Development of a real time polymerase chain reaction assay for equine encephalosis virus. J Virol Methods. 2014;195:205–10. DOIPubMedGoogle Scholar
  6. Aharonson-Raz  K, Steinman  A, Bumbarov  V, Maan  S, Maan  NS, Nomikou  K, et al. Isolation and phylogenetic grouping of equine encephalosis virus in Israel. Emerg Infect Dis. 2011;17:1883–6. DOIPubMedGoogle Scholar
  7. Yozwiak  NL, Skewes-Cox  P, Stenglein  MD, Balmaseda  A, Harris  E, DeRisi  JL. Virus identification in unknown tropical febrile illness cases using deep sequencing. PLoS Negl Trop Dis. 2012;6:e1485. DOIPubMedGoogle Scholar
  8. Prachayangprecha  S, Schapendonk  CM, Koopmans  MP, Osterhaus  AD, Schürch  AC, Pas  SD, et al. Exploring the potential of next-generation sequencing in detection of respiratory viruses. J Clin Microbiol. 2014;52:3722–30. DOIPubMedGoogle Scholar
  9. Shirato  K, Miyoshi  H, Kariwa  H, Takashima  I. Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses. J Virol Methods. 2005;126:119–25. DOIPubMedGoogle Scholar
  10. Reed  LJ, Muench  H. A simple method of estimating fifty per cent endpoints. Am J Hyg. 1938;27:493–7.
  11. Pyle  RL. Taxonomer: a relational data model for managing information relevant to taxonomic research. PhyloInformatics. 2004;1:1–54.

Main Article

Page created: April 17, 2018
Page updated: April 17, 2018
Page reviewed: April 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external