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Volume 24, Number 8—August 2018
Research

Susceptibility of Human Prion Protein to Conversion by Chronic Wasting Disease Prions

Marcelo A. BarriaComments to Author , Adriana Libori, Gordon Mitchell, and Mark W. Head
Author affiliations: National CJD Research and Surveillance Unit, University of Edinburgh, Edinburgh, Scotland, UK (M.A. Barria, A. Libori, M.W. Head); National and OIE Reference Laboratory for Scrapie and CWD, Canadian Food Inspection Agency, Ottawa, Ontario, Canada (G. Mitchell)

Main Article

Figure 5

Schematic representation of the partial purification of misfolded, disease-associated prion protein from chronic wasting disease (CWD)–affected deer brain specimens and its continued ability to seed the conversion of human prion protein (PrP) during protein misfolding cyclic amplification (PMCA) reactions. We normalized PrP, partially purified by detergent insolubility from reindeer and white-tailed deer CWD specimens, by using protease-resistant prion protein (PrPres) and subjected PrP to a sin

Figure 5. Schematic representation of the partial purification of misfolded, disease-associated prion protein from chronic wasting disease (CWD)–affected deer brain specimens and its continued ability to seed the conversion of human prion protein (PrP) during protein misfolding cyclic amplification (PMCA) reactions. We normalized PrP, partially purified by detergent insolubility from reindeer and white-tailed deer CWD specimens, by using protease-resistant prion protein (PrPres) and subjected PrP to a single round of PMCA in humanized transgenic PRNP codon 129 homozygous methionine. We performed Western blot analysis by using mAb 3F4 (for detection of human PrPres) and mAb 6H4 (for detection of CWD PrPres and human PrPres). Molecular mass of electrophoretic markers is given. Odd and even number lanes show reaction mixtures before and after PMCA. mAb, monoclonal antibody; PMCA, protein misfolding cyclic amplification; PrPc, normal isoform of the prion protein; PrPsc, disease-associated isoform of the prion protein.

Main Article

Page created: July 17, 2018
Page updated: July 17, 2018
Page reviewed: July 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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