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Volume 24, Number 8—August 2018
Research

Susceptibility of Human Prion Protein to Conversion by Chronic Wasting Disease Prions

Marcelo A. BarriaComments to Author , Adriana Libori, Gordon Mitchell, and Mark W. Head
Author affiliations: National CJD Research and Surveillance Unit, University of Edinburgh, Edinburgh, Scotland, UK (M.A. Barria, A. Libori, M.W. Head); National and OIE Reference Laboratory for Scrapie and CWD, Canadian Food Inspection Agency, Ottawa, Ontario, Canada (G. Mitchell)

Main Article

Figure 1

Western blot analysis showing detectable levels of prion protein in the chronic wasting disease–affected cervid brain specimens used to evaluate the susceptibility of the human prion protein (PrP) to conversion by chronic wasting disease prions. We analyzed brain homogenate derived from elk, white-tailed deer, and reindeer specimens by using Western blot to evaluate levels of total PrP. We subjected 2 μL of each 10% brain homogenate sample to Western blot and assessed detection of total PrP by m

Figure 1. Western blot analysis showing detectable levels of prion protein in the chronic wasting disease–affected cervid brain specimens used to evaluate the susceptibility of the human prion protein (PrP) to conversion by chronic wasting disease prions. We analyzed brain homogenate derived from elk, white-tailed deer, and reindeer specimens by using Western blot to evaluate levels of total PrP. We subjected 2 μL of each 10% brain homogenate sample to Western blot and assessed detection of total PrP by mAb 6H4. We performed 3 technical repeats with similar results; a representative Western blot is shown. Reference molecular markers have been included. mAb, monoclonal antibody.

Main Article

Page created: July 17, 2018
Page updated: July 17, 2018
Page reviewed: July 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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