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Volume 25, Number 10—October 2019
Research

Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections

Nisreen M.A. Okba, V. Stalin Raj, Ivy Widjaja, Corine H. GeurtsvanKessel, Erwin de Bruin, Felicity D. Chandler, Wan Beom Park, Nam-Joong Kim, Elmoubasher A.B.A. Farag, Mohammed Al-Hajri, Berend-Jan Bosch, Myoung-don Oh, Marion P.G. Koopmans, Chantal B.E.M. Reusken, and Bart L. HaagmansComments to Author 
Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (N.M.A. Okba, V.S. Raj, C.H. GeurtsvanKessel, E. de Bruin, F.D. Chandler, M.P.G. Koopmans, C.B.E.M. Reusken, B.L. Haagmans); Utrecht University, Utrecht, the Netherlands (I. Widjaja, B.-J. Bosch); Seoul National University College of Medicine, Seoul, South Korea (W.B. Park, N.-J. Kim, M.-D. Oh); Ministry of Public Health, Doha, Qatar (E.A.B.A. Farag, M. Al-Hajri)

Main Article

Figure 5

Correlation between neutralizing and S1 antibody responses and comparison of different S1 platforms. A) PRNT90 neutralization titers and IgG titers obtained by in-house S1 ELISA among PCR-confirmed MERS-CoV patients and persons with camel contact. Spearman correlation r value and 2-tailed p-value are shown. Yellow shading indicates S1-reactive nonneutralizing antibodies. B) Receiver operator characteristic (ROC) curves comparing the specificity and sensitivity of different MERS-CoV S1–based plat

Figure 5. Correlation between neutralizing and S1 antibody responses and comparison of different S1 platforms. A) PRNT90 neutralization titers and IgG titers obtained by in-house S1 ELISA among PCR-confirmed MERS-CoV patients and persons with camel contact. Spearman correlation r value and 2-tailed p-value are shown. Yellow shading indicates S1-reactive nonneutralizing antibodies. B) Receiver operator characteristic (ROC) curves comparing the specificity and sensitivity of different MERS-CoV S1–based platforms for the diagnosis of MERS-CoV infections among PCR-confirmed cases. AUC for iELISA (blue) is 1; for S1 microarray (red) is 0.9893; for rELISA (green) is 0.9481. Dotted lines show the cutoff for each assay. AUC, area under the curve; dpd, days postdiagnosis; PRNT90, 90% reduction in plaque reduction neutralization test.

Main Article

Page created: September 17, 2019
Page updated: September 17, 2019
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