Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 25, Number 4—April 2019
Research

Differences in Neuropathogenesis of Encephalitic California Serogroup Viruses

Alyssa B. Evans, Clayton W. Winkler, and Karin E. PetersonComments to Author 
Author affiliations: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA

Main Article

Figure 5

Cytotoxicity and viral replication kinetics assays of California serogroup (CSG) viruses in SH-SY5Y cells and human neural stem cells (hNSCs) for up to 96 hours postinoculation in study of neuropathogenesis. SH-SY5Y cells were infected at a multiplicity of infection of 0.1 (A, E, I) or 0.01 (B, F, J), and hNSCs were infected at a multiplicity of infection of 0.01 (C, G, K) or 0.001 (D, H, L). A–D) Confluence was measured over time on the IncuCyte (Essen Bioscience, https://www.essenbioscience.co

Figure 5. Cytotoxicity and viral replication kinetics assays of California serogroup (CSG) viruses in SH-SY5Y cells and human neural stem cells (hNSCs) for up to 96 hours postinoculation in study of neuropathogenesis. SH-SY5Y cells were infected at a multiplicity of infection of 0.1 (A, E, I) or 0.01 (B, F, J), and hNSCs were infected at a multiplicity of infection of 0.01 (C, G, K) or 0.001 (D, H, L). A–D) Confluence was measured over time on the IncuCyte (Essen Bioscience, https://www.essenbioscience.com) as the percentage of the image covered by cells. Graphs show the percentage of confluence compared with that of uninfected control wells. E–H) Cell death was measured over time with the IncuCyte and reported as the total integrated fluorescent intensity of the Cytotox Green (Essen Bioscience) reagent. I–L) Supernatants were harvested from SH-SY5Y cells and hNSCs at 1, 6, 12, 24, 48, 72, and 96 hours postinfection and titered on Vero cells by plaque assay. All error bars indicate SEM. PFU, plaque-forming units.

Main Article

Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external