Volume 25, Number 5—May 2019
Research
Lassa Virus Targeting of Anterior Uvea and Endothelium of Cornea and Conjunctiva in Eye of Guinea Pig Model
Table
Guinea pig | Age at D0/sex | dpi | Viral RNA† | IHC distribution | IHC score | H&E |
---|---|---|---|---|---|---|
Jos-1‡ | 3 y 10 mo/F | 14 | 4.55 × 105 | Conjunctival endothelium and anterior uvea; eyelid epithelium and endothelium | +++/ ++++ | Mild conjunctivitis and anterior uveitis |
Jos-2 | 3 y 10 mo/M | 41 | 2.50 × 101 | None | − | Mild perivascular mononuclear inflammation, sclera |
Jos-3‡ | 2 y 5 mo/F | 20 | 1.32 × 105 | Anterior uvea, peripheral corneal endothelium | ++ | Neovascularization at corneal margin, mild conjunctivitis |
Jos-4‡ | 2 y 4 mo/F | 17 | 7.87 × 105 | Endothelium, conjunctiva, and peripheral cornea; anterior uvea | +++ | Neovascularization at corneal margin, mild conjunctivitis |
Jos-5‡ | 0 y 6 mo/M | 17 | 1.02 × 106 | Anterior uvea | ++ | Mild anterior uveitis |
Jos-6‡ | 0 y 6 mo/M | 18 | 5.32 × 105 | Anterior uvea | + | Mild neovascularization at corneal margin |
Jos-7‡ | 3 y 7 mo/F | 23 | 5.23 × 106 | Conjunctival endothelium and anterior uvea | ++ | Neovascularization at corneal margin, mild conjunctivitis, anterior uveitis |
Jos-8‡ | 3 y 3 mo/F | 21 | 2.02 × 106 | Anterior uvea, peripheral corneal endothelium | ++ | Neovascularization at corneal margin, anterior uveitis |
Jos-9‡ | 3 y 6 mo/M | 20 | 6.71 × 106 | Conjunctival and peripheral corneal endothelium and anterior uvea, eyelid and lacrimal gland | +++ | Neovascularization at corneal margin, moderate conjunctivitis, anterior uveitis |
Jos-10‡ | >2 y/M | 23 | 2.26 × 106 | Conjunctival and peripheral corneal endothelium and anterior uvea | ++ | Mild neovascularization at corneal margin, minimal anterior uveitis |
NJ2015-1 | 3 y 11 mo/F | 41 | BLD | None | − | Minimal heterophilic infiltrate at corneal margin |
NJ2015-2 | 3 y 8 mo/M | 41 | BLD | None | − | NSF |
NJ2015-3 | 2 y 5 mo/F | 41 | BLD | None | − | NSF |
NJ2015-4 | 2 y 4 mo/F | 41 | BLD | None | − | NSF |
NJ2015-5 | 0 y 6 mo/M | 41 | 1.54 × 102 | None | − | Minimal heterophilic infiltrate at corneal margin, minimal chronic anterior uveitis |
NJ2015-6 | 0 y 6 mo/M | 41 | 4.28 × 102 | None | − | Mild anterior uveitis |
*All survivors were euthanized at end of study (41 dpi). BLD, below limit of detection (<5 copies); D0, day 0; dpi, days postinfection; H&E, hematoxylin and eosin staining; IHC, immunohistochemical; Jos, guinea pig infected with recombinant LASV-Josiah; LASV, Lassa virus; NJ2015, guinea pig infected with recombinant LASV 812673-LBR-USA-2015; NSF, no significant findings; −, negative results at staining; +, mild immunostaining; ++, moderate immunostaining; +++/++++, abundant immunostaining.
†LASV small segment copies in the eye quantified per millileter of eluted RNA.
‡Guinea pig euthanized because of disease.
Page created: April 18, 2019
Page updated: April 18, 2019
Page reviewed: April 18, 2019
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