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Volume 25, Number 5—May 2019
Research

Lassa Virus Targeting of Anterior Uvea and Endothelium of Cornea and Conjunctiva in Eye of Guinea Pig Model

Joy M. Gary, Stephen R. Welch, Jana M. Ritter, JoAnn Coleman-McCray, Thanhthao Huynh, Markus H. Kainulainen, Brigid C. Bollweg, Vaunita Parihar, Stuart T. Nichol, Sherif R. Zaki, Christina F. Spiropoulou, and Jessica R. SpenglerComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table

Summary of ocular LASV staining and histopathologic findings in LASV-infected guinea pigs in study of LASV targeting of anterior uvea and endothelium of cornea and conjunctiva in eye*

Guinea pig Age at D0/sex dpi Viral RNA† IHC distribution IHC score H&E
Jos-1‡ 3 y 10 mo/F 14 4.55 × 105 Conjunctival endothelium and anterior uvea; eyelid epithelium and endothelium +++/
++++ Mild conjunctivitis and anterior uveitis
Jos-2 3 y 10 mo/M 41 2.50 × 101 None − Mild perivascular mononuclear inflammation, sclera
Jos-3‡ 2 y 5 mo/F 20 1.32 × 105 Anterior uvea, peripheral corneal endothelium ++ Neovascularization at corneal margin, mild conjunctivitis
Jos-4‡ 2 y 4 mo/F 17 7.87 × 105 Endothelium, conjunctiva, and peripheral cornea; anterior uvea +++ Neovascularization at corneal margin, mild conjunctivitis
Jos-5‡ 0 y 6 mo/M 17 1.02 × 106 Anterior uvea ++ Mild anterior uveitis
Jos-6‡ 0 y 6 mo/M 18 5.32 × 105 Anterior uvea + Mild neovascularization at corneal margin
Jos-7‡ 3 y 7 mo/F 23 5.23 × 106 Conjunctival endothelium and anterior uvea ++ Neovascularization at corneal margin, mild conjunctivitis, anterior uveitis
Jos-8‡ 3 y 3 mo/F 21 2.02 × 106 Anterior uvea, peripheral corneal endothelium ++ Neovascularization at corneal margin, anterior uveitis
Jos-9‡ 3 y 6 mo/M 20 6.71 × 106 Conjunctival and peripheral corneal endothelium and anterior uvea, eyelid and lacrimal gland +++ Neovascularization at corneal margin, moderate conjunctivitis, anterior uveitis
Jos-10‡ >2 y/M 23 2.26 × 106 Conjunctival and peripheral corneal endothelium and anterior uvea ++ Mild neovascularization at corneal margin, minimal anterior uveitis
NJ2015-1 3 y 11 mo/F 41 BLD None − Minimal heterophilic infiltrate at corneal margin
NJ2015-2 3 y 8 mo/M 41 BLD None − NSF
NJ2015-3 2 y 5 mo/F 41 BLD None − NSF
NJ2015-4 2 y 4 mo/F 41 BLD None − NSF
NJ2015-5 0 y 6 mo/M 41 1.54 × 102 None − Minimal heterophilic infiltrate at corneal margin, minimal chronic anterior uveitis
NJ2015-6 0 y 6 mo/M 41 4.28 × 102 None − Mild anterior uveitis

*All survivors were euthanized at end of study (41 dpi). BLD, below limit of detection (<5 copies); D0, day 0; dpi, days postinfection; H&E, hematoxylin and eosin staining; IHC, immunohistochemical; Jos, guinea pig infected with recombinant LASV-Josiah; LASV, Lassa virus; NJ2015, guinea pig infected with recombinant LASV 812673-LBR-USA-2015; NSF, no significant findings; −, negative results at staining; +, mild immunostaining; ++, moderate immunostaining; +++/++++, abundant immunostaining.
†LASV small segment copies in the eye quantified per millileter of eluted RNA.
‡Guinea pig euthanized because of disease.

Main Article

Page created: April 18, 2019
Page updated: April 18, 2019
Page reviewed: April 18, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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