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Volume 25, Number 6—June 2019
Synopsis

Immunopathology of Fatal Human Variegated Squirrel Bornavirus 1 Encephalitis, Germany, 2011–2013

Dennis TappeComments to Author , Jonas Schmidt-Chanasit, Jessica Rauch, Petra Allartz, and Christiane Herden
Author affiliations: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (D. Tappe, J. Schmidt-Chanasit, J. Rauch, P. Allartz); German Centre for Infection Research, Hamburg (J. Schmidt-Chanasit); University of Giessen, Giessen, Germany (C. Herden)

Main Article

Table 2

Real-time RT-PCR results for variegated squirrel bornavirus 1 RNA in different brain areas and internal organs, Germany*

Sample Cycle threshold
Patient 1 Patient 2 Patient 3
Cortical biopsy 31.2 30.8 NA
Cortex frontal NA 31.1 29.9
Cortex temporal NA 27.9 28.9
Striatum NA 28.9 29.7
Hippocampus NA 28.3 29.4
Midbrain/substantia nigra NA 26.4 29.4
Cerebellum NA 34.6 29.9
Olfactory bulb NA NA 29.7
Myocardium NA Neg Neg
Lungs NA Neg Neg
Kidney NA Neg Neg
Liver NA Neg Neg
Spleen NA Neg Neg

*All materials tested were formalin-fixed paraffin-embedded tissue specimens. Not all tissues were available from all patients because no autopsy was performed on patient 1 and no biopsy sample was taken from patient 3. From patient 4, real-time RT-PCR analyses for variegated squirrel bornavirus 1 RNA in different brain areas and internal organs were performed earlier (2). NA, not available; neg, negative; RT-PCR, reverse transcription PCR.

Main Article

References
  1. Hoffmann  B, Tappe  D, Höper  D, Herden  C, Boldt  A, Mawrin  C, et al. A variegated squirrel bornavirus associated with fatal human encephalitis. N Engl J Med. 2015;373:154–62. DOIPubMed
  2. Tappe  D, Schlottau  K, Cadar  D, Hoffmann  B, Balke  L, Bewig  B, et al. Occupation-associated fatal limbic encephalitis caused by variegated squirrel bornavirus 1, Germany, 2013. Emerg Infect Dis. 2018;24:978–87. DOIPubMed
  3. Schlottau  K, Hoffmann  B, Homeier-Bachmann  T, Fast  C, Ulrich  RG, Beer  M, et al. Multiple detection of zoonotic variegated squirrel bornavirus 1 RNA in different squirrel species suggests a possible unknown origin for the virus. Arch Virol. 2017;162:2747–54. DOIPubMed
  4. Schlottau  K, Jenckel  M, van den Brand  J, Fast  C, Herden  C, Höper  D, et al. Variegated squirrel bornavirus 1 in squirrels, Germany and the Netherlands. Emerg Infect Dis. 2017;23:477–81. DOIPubMed
  5. Carbone  KM, Duchala  CS, Griffin  JW, Kincaid  AL, Narayan  O. Pathogenesis of Borna disease in rats: evidence that intra-axonal spread is the major route for virus dissemination and the determinant for disease incubation. J Virol. 1987;61:3431–40.PubMed
  6. Herden  C, Herzog  S, Richt  JA, Nesseler  A, Christ  M, Failing  K, et al. Distribution of Borna disease virus in the brain of rats infected with an obesity-inducing virus strain. Brain Pathol. 2000;10:39–48. DOIPubMed
  7. Ludlow  M, Kortekaas  J, Herden  C, Hoffmann  B, Tappe  D, Trebst  C, et al. Neurotropic virus infections as the cause of immediate and delayed neuropathology. Acta Neuropathol. 2016;131:159–84. DOIPubMed
  8. Stitz  L, Bilzer  T, Planz  O. The immunopathogenesis of Borna disease virus infection. Front Biosci. 2002;7:d541–55. DOIPubMed
  9. Berger  JR, Aksamit  AJ, Clifford  DB, Davis  L, Koralnik  IJ, Sejvar  JJ, et al. PML diagnostic criteria: consensus statement from the AAN Neuroinfectious Disease Section. Neurology. 2013;80:1430–8. DOIPubMed
  10. Lipkin  WI, Briese  T, Hornig  M. Borna disease virus - fact and fantasy. Virus Res. 2011;162:162–72. DOIPubMed
  11. Tizard  I, Ball  J, Stoica  G, Payne  S. The pathogenesis of bornaviral diseases in mammals. Anim Health Res Rev. 2016;17:92–109. DOIPubMed
  12. Rott  R, Herzog  S, Richt  J, Stitz  L. Immune-mediated pathogenesis of Borna disease. Zentralbl Bakteriol Mikrobiol Hyg A. 1988;270:295–301. DOIPubMed
  13. Kramer  K, Schaudien  D, Eisel  UL, Herzog  S, Richt  JA, Baumgärtner  W, et al. TNF-overexpression in Borna disease virus-infected mouse brains triggers inflammatory reaction and epileptic seizures. PLoS One. 2012;7:e41476. DOIPubMed
  14. Bilzer  T, Planz  O, Lipkin  WI, Stitz  L. Presence of CD4+ and CD8+ T cells and expression of MHC class I and MHC class II antigen in horses with Borna disease virus-induced encephalitis. Brain Pathol. 1995;5:223–30. DOIPubMed
  15. Caplazi  P, Ehrensperger  F. Spontaneous Borna disease in sheep and horses: immunophenotyping of inflammatory cells and detection of MHC-I and MHC-II antigen expression in Borna encephalitis lesions. Vet Immunol Immunopathol. 1998;61:203–20. DOIPubMed
  16. Hausmann  J, Hallensleben  W, de la Torre  JC, Pagenstecher  A, Zimmermann  C, Pircher  H, et al. T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein. Proc Natl Acad Sci U S A. 1999;96:9769–74. DOIPubMed
  17. Khan  S, Ahmad  K, Alshammari  EM, Adnan  M, Baig  MH, Lohani  M, et al. Implication of caspase-3 as a common therapeutic target for multineurodegenerative disorders and its inhibition using nonpeptidyl natural compounds. BioMed Res Int. 2015;2015:379817. DOIPubMed
  18. Sun  Y, Xu  Y, Geng  L. Caspase-3 inhibitor prevents the apoptosis of brain tissue in rats with acute cerebral infarction. Exp Ther Med. 2015;10:133–8. DOIPubMed
  19. Williams  BL, Hornig  M, Yaddanapudi  K, Lipkin  WI. Hippocampal poly(ADP-Ribose) polymerase 1 and caspase 3 activation in neonatal bornavirus infection. J Virol. 2008;82:1748–58. DOIPubMed
  20. Weissenböck  H, Hornig  M, Hickey  WF, Lipkin  WI. Microglial activation and neuronal apoptosis in Bornavirus infected neonatal Lewis rats. Brain Pathol. 2000;10:260–72. DOIPubMed
  21. Han  BH, Xu  D, Choi  J, Han  Y, Xanthoudakis  S, Roy  S, et al. Selective, reversible caspase-3 inhibitor is neuroprotective and reveals distinct pathways of cell death after neonatal hypoxic-ischemic brain injury. J Biol Chem. 2002;277:30128–36. DOIPubMed
  22. Mizutani  T, Inagaki  H, Tada  M, Hayasaka  D, Murphy  M, Fujiwara  T, et al. The mechanism of actinomycin D-mediated increase of Borna disease virus (BDV) RNA in cells persistently infected by BDV. Microbiol Immunol. 2000;44:597–603. DOIPubMed
  23. Hausmann  J, Schamel  K, Staeheli  P. CD8(+) T lymphocytes mediate Borna disease virus-induced immunopathology independently of perforin. J Virol. 2001;75:10460–6. DOIPubMed
  24. Zhang  G, Kobayashi  T, Kamitani  W, Komoto  S, Yamashita  M, Baba  S, et al. Borna disease virus phosphoprotein represses p53-mediated transcriptional activity by interference with HMGB1. J Virol. 2003;77:12243–51. DOIPubMed
  25. Delbue  S, Comar  M, Ferrante  P. Review on the role of the human Polyomavirus JC in the development of tumors. Infect Agent Cancer. 2017;12:10. DOIPubMed
  26. Ariza  A, Mate  JL, Isamat  M, Calatrava  A, Fernández-Vasalo  A, Navas-Palacios  JJ. Overexpression of Ki-67 and cyclins A and B1 in JC virus-infected cells of progressive multifocal leukoencephalopathy. J Neuropathol Exp Neurol. 1998;57:226–30. DOIPubMed

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CrossRef reports the first page should be "A793", not "d541". (Ref. 8 "Stitz, Bilzer, Planz, 2002")

CrossRef reports the first page should be "1", not "379817". (Ref. 17 "Khan, Ahmad, Alshammari, Adnan, Baig, Lohani, et al., 2015")

Page created: May 20, 2019
Page updated: May 20, 2019
Page reviewed: May 20, 2019
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