Volume 25, Number 6—June 2019
Synopsis
Immunopathology of Fatal Human Variegated Squirrel Bornavirus 1 Encephalitis, Germany, 2011–2013
Table 2
Real-time RT-PCR results for variegated squirrel bornavirus 1 RNA in different brain areas and internal organs, Germany*
Sample |
Cycle threshold
|
||
---|---|---|---|
Patient 1 | Patient 2 | Patient 3 | |
Cortical biopsy | 31.2 | 30.8 | NA |
Cortex frontal | NA | 31.1 | 29.9 |
Cortex temporal | NA | 27.9 | 28.9 |
Striatum | NA | 28.9 | 29.7 |
Hippocampus | NA | 28.3 | 29.4 |
Midbrain/substantia nigra | NA | 26.4 | 29.4 |
Cerebellum | NA | 34.6 | 29.9 |
Olfactory bulb | NA | NA | 29.7 |
Myocardium | NA | Neg | Neg |
Lungs | NA | Neg | Neg |
Kidney | NA | Neg | Neg |
Liver | NA | Neg | Neg |
Spleen | NA | Neg | Neg |
*All materials tested were formalin-fixed paraffin-embedded tissue specimens. Not all tissues were available from all patients because no autopsy was performed on patient 1 and no biopsy sample was taken from patient 3. From patient 4, real-time RT-PCR analyses for variegated squirrel bornavirus 1 RNA in different brain areas and internal organs were performed earlier (2). NA, not available; neg, negative; RT-PCR, reverse transcription PCR.
References
- Carbone KM, Duchala CS, Griffin JW, Kincaid AL, Narayan O. Pathogenesis of Borna disease in rats: evidence that intra-axonal spread is the major route for virus dissemination and the determinant for disease incubation. J Virol. 1987;61:3431–40.PubMed
CrossRef reports the first page should be "A793", not "d541". (Ref. 8 "Stitz, Bilzer, Planz, 2002")
CrossRef reports the first page should be "1", not "379817". (Ref. 17 "Khan, Ahmad, Alshammari, Adnan, Baig, Lohani, et al., 2015")