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Volume 26, Number 10—October 2020
Dispatch

Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Clinton R. Paden1, Ying Tao1, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, and Suxiang TongComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Paden, Y. Tao, K. Queen, Y. Li, S. Tong); IHRC, Atlanta (J. Zhang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara)

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Figure 2

Sequencing of severe acute respiratory syndrome coronavirus 2 clinical samples. A, B) Percentage mapped (A) and percentage genome coverage (B) for 167 clinical severe acute respiratory syndrome coronavirus 2 samples amplified by using a multiplex PCR strategy and sequenced on the MinION apparatus (https://nanoporetech.com). C) Time-lapse of 20× genome coverage obtained for clinical specimens at the indicated cycle threshold values. Data points indicate average coverage over time for various samp

Figure 2. Sequencing of severe acute respiratory syndrome coronavirus 2 clinical samples. A, B) Percentage mapped (A) and percentage genome coverage (B) for 167 clinical severe acute respiratory syndrome coronavirus 2 samples amplified by using a multiplex PCR strategy and sequenced on the MinION apparatus (https://nanoporetech.com). C) Time-lapse of 20× genome coverage obtained for clinical specimens at the indicated cycle threshold values. Data points indicate average coverage over time for various samples and grouped by run and the indicated Ct values. Ct, cycle threshold; N2, nucleoprotein 2.

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1These authors contributed equally to this article.

Page created: June 23, 2020
Page updated: September 17, 2020
Page reviewed: September 17, 2020
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