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Volume 26, Number 10—October 2020
Dispatch

Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Clinton R. Paden1, Ying Tao1, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, and Suxiang TongComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Paden, Y. Tao, K. Queen, Y. Li, S. Tong); IHRC, Atlanta (J. Zhang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara)

Main Article

Table 1

Genome consensus accuracy for sequencing severe acute respiratory syndrome coronavirus 2*

Virus titer (cycle threshold) % Coverage, 20׆ Indels Indel bases Single-nucleotide polymorphisms % Identity†
22.3 99.659 0 0 0 100
99.722 0 0 0 100
99.635
 0
 0
 0
 100
25.7 99.635 0 0 0 100
99.615 0 0 0 100
99.642
 0
 0
 0
 100
29.2 99.508 0 0 0 100
99.635 0 0 0 100
99.615
 0
 0
 0
 100
33.2 93.024 1 1 0 100
93.603 2 35 0 100
87.894
 0
 0
 0
 100
35.6 41.653 1 1 0 100
51.266 0 0 1 99.993
50.821
 1
 15
 2
 99.987
37.6 14.634 0 0 1 99.977
9.317 0 0 0 100
12.363 0 0 0 100

*Because the 5′ and 3′ ends are primer sequences, 100% coverage is not possible.
†Percentage of covered bases identical to reference sequence, excludes indels and low-coverage bases.

Main Article

1These authors contributed equally to this article.

Page created: June 23, 2020
Page updated: September 17, 2020
Page reviewed: September 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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