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Volume 26, Number 10—October 2020
Dispatch

Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Clinton R. Paden1, Ying Tao1, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, and Suxiang TongComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Paden, Y. Tao, K. Queen, Y. Li, S. Tong); IHRC, Atlanta (J. Zhang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara)

Main Article

Table 2

Comparison of input, time, and cost requirements for sequencing 1 or 96 specimens of severe acute respiratory syndrome coronavirus 2

Method Input, μL* 1 sample
 96 samples
Turnaround time Approximate cost/sample† Turnaround time Approximate cost/sample†
Multiplex/MinION 10 6–8 h $528.70 8–10 h $35.88
Multiplex/MiSeq 10 30–68 h‡ $1,443.29 30–68 h‡ $57.87
Singleplex/Sanger 190 16–18 h $354.40 17–19 d $354.40

*Assumes a process with 200 μL of resuspended respiratory specimen (from a total of 2 mL), extracted, and eluted into 100 μL See Appendix for details.
†Includes specific enzyme and reagent costs; excludes common laboratory supplies and labor costs.
‡Varies according to the sequencing kit used.

Main Article

1These authors contributed equally to this article.

Page created: June 23, 2020
Page updated: September 17, 2020
Page reviewed: September 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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