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Volume 26, Number 12—December 2020
Dispatch

Sensitive Detection of SARS-CoV-2–Specific Antibodies in Dried Blood Spot Samples

Gabriella L. Morley, Stephen Taylor, Sian Jossi, Marisol Perez-Toledo, Sian E. Faustini, Edith Marcial-Juarez, Adrian M. Shields, Margaret Goodall, Joel D. Allen, Yasunori Watanabe, Maddy L. Newby, Max Crispin, Mark T. Drayson, Adam F. Cunningham, Alex G. Richter1, and Matthew K. O’Shea1Comments to Author 
Author affiliations: University of Birmingham, Birmingham, UK (G.L. Morley, S. Taylor, S. Jossi, M. Perez-Toledo, S.E. Faustini, E. Marcial-Juarez, A.M. Shields, M. Goodall, M.T. Drayson, A.F. Cunningham, A.G. Richter, M.K. O’Shea); The Saving Lives Charity, Birmingham (S. Taylor); University Hospitals Birmingham NHS Foundation Trust, Birmingham (S. Taylor, A.M. Shields, A.G. Richter, M.K. O’Shea); University of Southampton, Southampton, UK (J.D. Allen, Y. Watanabe, M.L. Newby, M. Crispin); University of Oxford, Oxford, UK (Y. Watanabe)

Main Article

Figure 1

Elution of SARS-CoV-2 anti-spike glycoprotein antibodies from DBS samples, showing 3-fold DBS eluate (A) (initial 1:3 dilution) and serum (B) (initial 1:15 dilution) titrations. Dashed line indicates pre–August 2019 DBS samples (n = 11). Red circles indicate PCR-positive samples (n = 5). Black circles indicate PCR-unknown samples (n = 11), from matched contemporaneous samples. All samples were selected at random for inclusion. DBS, dried blood spot; OD450, optical density at 450 nm; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Figure 1. Elution of SARS-CoV-2 anti-spike glycoprotein antibodies from DBS samples, showing 3-fold DBS eluate (A) (initial 1:3 dilution) and serum (B) (initial 1:15 dilution) titrations. Dashed line indicates pre–August 2019 DBS samples (n = 11). Red circles indicate PCR-positive samples (n = 5). Black circles indicate PCR-unknown samples (n = 11), from matched contemporaneous samples. All samples were selected at random for inclusion. DBS, dried blood spot; OD450, optical density at 450 nm; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Main Article

1These senior authors contributed equally to this article.

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