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Volume 26, Number 12—December 2020
Dispatch

Sensitive Detection of SARS-CoV-2–Specific Antibodies in Dried Blood Spot Samples

Gabriella L. Morley, Stephen Taylor, Sian Jossi, Marisol Perez-Toledo, Sian E. Faustini, Edith Marcial-Juarez, Adrian M. Shields, Margaret Goodall, Joel D. Allen, Yasunori Watanabe, Maddy L. Newby, Max Crispin, Mark T. Drayson, Adam F. Cunningham, Alex G. Richter1, and Matthew K. O’Shea1Comments to Author 
Author affiliations: University of Birmingham, Birmingham, UK (G.L. Morley, S. Taylor, S. Jossi, M. Perez-Toledo, S.E. Faustini, E. Marcial-Juarez, A.M. Shields, M. Goodall, M.T. Drayson, A.F. Cunningham, A.G. Richter, M.K. O’Shea); The Saving Lives Charity, Birmingham (S. Taylor); University Hospitals Birmingham NHS Foundation Trust, Birmingham (S. Taylor, A.M. Shields, A.G. Richter, M.K. O’Shea); University of Southampton, Southampton, UK (J.D. Allen, Y. Watanabe, M.L. Newby, M. Crispin); University of Oxford, Oxford, UK (Y. Watanabe)

Main Article

Figure 2

Effectiveness of DBS sampling for SARS-CoV-2 anti-spike glycoprotein detection. A) Correlation between matched DBS eluate (1:10) and serum (1:100) OD450 ELISA results (n = 87). Red circles indicate PCR-positive samples (n = 31). Black circles indicate PCR-unknown samples (n = 56). B) Bland-Altman mean-difference comparison of DBS eluate (1:10) and serum (1:100) OD450 ELISA results (dashed lines indicate 95% limits of agreement [−0.281 to 0.504]). DBS, dried blood spot; OD450, optical density at 450 nm; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Figure 2. Effectiveness of DBS sampling for SARS-CoV-2 anti-spike glycoprotein detection. A) Correlation between matched DBS eluate (1:10) and serum (1:100) OD450 ELISA results (n = 87). Red circles indicate PCR-positive samples (n = 31). Black circles indicate PCR-unknown samples (n = 56). B) Bland-Altman mean-difference comparison of DBS eluate (1:10) and serum (1:100) OD450 ELISA results (dashed lines indicate 95% limits of agreement [−0.281 to 0.504]). DBS, dried blood spot; OD450, optical density at 450 nm; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Main Article

1These senior authors contributed equally to this article.

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