Volume 26, Number 2—February 2020
Research
Novel Subclone of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 11 with Enhanced Virulence and Transmissibility, China
Figure 2
![Phylogenetic analysis of 216 CRKP ST11 isolates, China, 2013–2017, including 154 CRKP isolates collected during 2012–2017 in study of bloodstream infections in a tertiary hospital and 62 isolates that were sequenced in previous studies (Appendix 2 Table 1). The phylogenetic tree was obtained by mapping all sequence reads to the hybrid assembly of KP47434 and removing the recombined regions from the alignment. The tree was rooted using ST1731 isolate EuSCAPE_ES29 (ERR1541319), which was included](/eid/images/19-0594-F2.jpg)
Figure 2. Phylogenetic analysis of 216 CRKP ST11 isolates, China, 2013–2017, including 154 CRKP isolates collected during 2012–2017 in study of bloodstream infections in a tertiary hospital and 62 isolates that were sequenced in previous studies (Appendix 2 Table 1). The phylogenetic tree was obtained by mapping all sequence reads to the hybrid assembly of KP47434 and removing the recombined regions from the alignment. The tree was rooted using ST1731 isolate EuSCAPE_ES29 (ERR1541319), which was included in this analysis but later removed from the tree (a tree including this outgroup is shown in Appendix 1 Figure 1). Five capsular types (KL31, KL47, KL64, KL103, and KL105) were detected in our ST11 collection, which are indicated in different colors as shown in the legend. Some of virulence genes detected are shown here. The rmpA2 gene carried by KL64 isolates was frameshifted, namely rmpA2*. Aerobactin and salmochelin represent the iucABCD-iutA and iroBCDN gene clusters, respectively. The fatal outbreak clone reported in China recently (12) is highlighted on the tree. Lanes: 1, year; 2, country; 3, K-type; 4, rmpA; 5, rmpA2; 6, aerobactin; 7, peg-344; 8, salmochelin; 9, blaKPC. Scale bar indicates single-nucleotide polymorphisms. CRKP, carbapenem-resistant Klebsiella pneumoniae; KL, capsular loci; ST, sequence type.
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1These first authors contributed equally to this article.
2These senior authors contributed equally to this article.