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Volume 26, Number 3—March 2020
Research

Stable and Local Reservoirs of Mycobacterium ulcerans Inferred from the Nonrandom Distribution of Bacterial Genotypes, Benin

Clément Coudereau, Alban Besnard, Marie Robbe-Saule, Céline Bris, Marie Kempf, Roch Christian Johnson, Télésphore Yao Brou, Ronald Gnimavo, Sara Eyangoh, Fida Khater, and Estelle MarionComments to Author 
Author affiliations: Université d’Angers, Angers, France (C. Coudereau, A. Besnard, M. Robbe-Saule, M. Kempf, F. Khater, E. Marion); INSERM, Angers (C. Coudereau, A. Besnard, M. Robbe-Saule, M. Kempf, F. Khater, E. Marion); Centre Hospitalo-Universitaire d’Angers, Angers (C. Bris, M. Kempf); Université d'Abomey Calavi, Abomey Calavi, Benin (R.C. Johnson); Fondation Raoul Follereau, Paris, France (R.C. Johnson); Maison de la Télédétection, Montpellier, France (T.Y. Brou); Centre de Diagnostic et Traitement de la Lèpre et de l’Ulcère de Buruli, Pobè, Bénin (R. Gnimavo); International Pasteur Institute Network, Yaoundé, Cameroon (S. Eyangoh)

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Figure 3

Eight genotypes emerging from phylogenetic analysis of Mycobacterium ulcerans isolates from Buruli ulcer patients in Benin and Nigeria. This rooted circular phylogenetic tree was built by using PhyML (22) on the basis of the core alignment of all single-nucleotide polymorphisms obtained with Snippy 3.2 (19). The bootstraps values are only represented on primitive branches. Branches with bootstrap values <70% were collapsed as polytomies. The outgroup (Papua New Guinea genomes) and the referen

Figure 3. Eight genotypes emerging from phylogenetic analysis of Mycobacterium ulcerans isolates from Buruli ulcer patients in Benin and Nigeria. This rooted circular phylogenetic tree was built by using PhyML (22) on the basis of the core alignment of all single-nucleotide polymorphisms obtained with Snippy 3.2 (19). The bootstrap values are only represented on primitive branches. Branches with bootstrap values <70% were collapsed as polytomies. The outgroup (Papua New Guinea genomes) and the reference genome (Agy99) are not represented (see Appendix 1 Figure 1). On the basis of the segregation indicated by this tree, the genomes were divided in 8 genotypes, which are either monophyletic or paraphyletic. Each taxon was assigned a specific color. Subgenotypes of genotype 8 also are indicated. Scale bar indicates the Nei genetic distance.

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Page updated: February 20, 2020
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