Volume 27, Number 5—May 2021
Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands
|Animal||Cohort||SARS-CoV-2 S1 ELISA†||SARS-CoV-2 RBD ELISA†||VNT‡||No. samples||Result|
||SARS-CoV-2 exposed, n = 44||+||+||+||10||Seropositive|
|2020, n = 500
|Dog||SARS-CoV-2 exposed, n = 1||+||+||+||1||Seropositive|
|2020, n = 500||+||+||+||1||Seropositive|
*NA, not applicable; RBD, receptor-binding domain; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; S1, spike protein subunit 1; VNT, virus neutralization titer; –, negative; +, positive. †An ELISA optical density value greater than or equal to the cutoff value of 0.4 is a positive result, and an ELISA OD value less than the cutoff value is a negative result. ‡Neutralization titers of samples were determined by using the reciprocal of the highest dilution that resulted in >50% reduction of luciferase activity in pseudovirus virus neutralization. A VNT greater than or equal to the cutoff value of 16 is a positive result, and a VNT less than the cutoff value is a negative result.