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Volume 28, Number 12—December 2022
Research

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. KohComments to Author , Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)

Main Article

Table 1

Horse samples and vaccine samples from Thailand used in characterization of affecting strain of AHSV by DIVA rRT-PCR*

No. Sample ID Sample type Source
Initial batch of samples (n = 7) reported in (9)
1 110983/63 Tissue homogenate Pak Chong, Nakhon-Ratchasima
2 111495/63 Whole blood Cha-am, Phetchaburi
3 111406/63 Tissue homogenate Ko Chan, Chonburi
4 111146/63 Whole blood Pak Chong, Nakhon-Ratchasima
5 112080/63 Whole blood Hua Hin, Prachuap Khiri Khan
6 111789/63 Whole blood Damnoen Saduak, Ratchaburi
7 111367/63 Tissue homogenate Hua Hin, Prachuap Khiri Khan
8 AHSV-1 OBP VACCINE 10−3 vaccine EURL (Spain)
9 AHSV-1 OBP VACCINE 10−4 vaccine EURL (Spain)
10
 AHSV-1 OBP VACCINE 10−5
 vaccine
 EURL (Spain)
Samples collected from clinically affected horses (n = 31) in this study
1 111146/63 Whole blood Nakhon-Ratchasima
2 111147/63-5 Whole blood Nakhon-Ratchasima
3 111147/63-19 Whole blood Nakhon-Ratchasima
4 111147/63-20 Whole blood Nakhon-Ratchasima
5 111147/63-21 Whole blood Nakhon-Ratchasima
6 111147/63–22 Whole blood Nakhon-Ratchasima
7 111162/63-A Whole blood Nakhon-Ratchasima
8 111162/63-B Whole blood Nakhon-Ratchasima
9 111164/63-1 Whole blood Nakhon-Ratchasima
10 111164/63-4 Whole blood Nakhon-Ratchasima
11 111367/63-B Tissue homogenate Prachuap Khiri Khan
12 111406/63-A Tissue homogenate Chonburi
13 111406/63-B Tissue homogenate Chonburi
14 111496/63 Whole blood Prachuap Khiri Khan
15 111790/63 Whole blood Phetchaburi
16 112080/63 Whole blood Prachuap Khiri Khan
17 112590/63 Whole blood Phetchaburi
18 112594/63 Whole blood Phetchaburi
19 112680/63 Whole blood Phetchaburi
20 113308/63 Whole blood Srakaew
21 113480/63 Whole blood Prachuap Khiri Khan
22 113481/63 Whole blood Prachuap Khiri Khan
23 113489/63 Whole blood Srakaew
24 113561/63-1 Whole blood Phetchaburi
25 113561/63-3 Whole blood Phetchaburi
26 113869/63 Whole blood Prachuap Khiri Khan
27 113870/63-11 Whole blood Prachuap Khiri Khan
28 113870/63-5 Whole blood Phetchaburi
29 113871/63-N Whole blood Phetchaburi
30 113871/63-5140 Whole blood Phetchaburi
31
 113908/63
 Whole blood
 Phetchaburi
Samples collected from vaccinated horses (n = 12) in this study
1 116187/63 Whole blood Nakhon-Ratchasima
2 122045/63 Whole blood Pathumthani
3 135560/63 Tissue homogenate Lopburi
4 137720/63 Tissue homogenate Rayong
5 118696/63 Whole blood Srakaew
6 120865/63 Whole blood Ratchaburi
7 136979/63 Whole blood Suphanburi
8 121673/63-A Whole blood Prachuap Khiri Khan
9 121673/63-B Whole blood Prachuap Khiri Khan
10 121673/63-C Whole blood Prachuap Khiri Khan
11 124916/63-A Whole blood Prachuap Khiri Khan
12 124916/63-B Whole blood Prachuap Khiri Khan

*AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; ID, identification; rRT-PCR, real-time reverse transcription PCR.

Main Article

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