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Volume 28, Number 12—December 2022
Research

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. KohComments to Author , Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)

Main Article

Table 2

Sequence and position of the AHSV-1 DIVA primer and probes used in study of development of PCR to characterize affecting strain of AHSV, Thailand*

Primer/probe Sequence, 5′ → 3′ Sense Position
VP5-DIVA-F 5′ AGCGTCGGATGCAAAGAAATC 3′ + 1335–1355
VP5-DIVA-P1 5′ FAM- ACTACAGCTGGTGCATAAC 3′ MGB + 1426–1444
VP5-DIVA-P2-vac 5′ FAM- TTTACAGTTGGTTCATAAT 3′ MGB + 1426–1444
VP5-DIVA-R 5′ AAGCGCGTTCATTATCGTCC 3′ – 1494–1513

*Position numbering is with reference to AHSV-1 (GenBank accession no. KT030334). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals.

Main Article

Page created: October 28, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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