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Volume 28, Number 12—December 2022
Research

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. KohComments to Author , Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)

Main Article

Table 4

Comparison of assay sensitivity among different AHSV assays*

Sample Method Limit of detection AUC Slope
AHSV-1 Thailand 110983/63
 AHSV VP7 rRT-PCR [10−5] 0.943
 −3.89
AHSV-1 VP2 Serotyping rRT-PCR [10−4]
AHSV-1 VP5 DIVA rRT-PCR 1
 [10−4]
AHSV-1 OBP LAV AHSV VP7 rRT-PCR [10−6] 0.900 −3.49
AHSV-1 VP2 Serotyping rRT-PCR [10−5]
AHSV-1 VP5 DIVA rRT-PCR 2 [10−5]

*The limit of detection was reported from the lowest observed amount of DNA amplified below the Ct <40 threshold. The AUC and slope values derived from the calibration curve are also listed. AHSV, African horse sickness virus; AUC, area under the receiver operating curve; Ct, cycle threshold; DIVA, Differentiating Infected from Vaccinated Animals; OBP LAV, Onderstepoort Biologic Products (https://www.obpvaccines.co.za) live attenuated vaccine; rRT-PCR, real-time reverse transcription PCR; VP, viral protein.

Main Article

Page created: October 28, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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