Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1
Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. Koh
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, Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)
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Figure 1
![Nucleotide sequence alignment of the VP5 gene for the OBP AHSV vaccine strains and Thailand AHSV-1 field isolate at the 1321–1560 region (numbering according to the OBP AHSV-1 isolate, GenBank accession no. KT030334) by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). OBP strain GenBank accession nos.: AHSV-1, KT030334; AHSV-3, KT030344; AHSV-4, KT030354. Thailand AHSV-1 isolate GenBank accession no.: MT711962. Yellow indicates the primer-binding regions (VP5-DIVA-F/R) and green the probe-binding region (VP5-DIVA-P1). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.](/eid/images/22-0594-F1.jpg)
Figure 1. Nucleotide sequence alignment of the VP5 gene for the OBP AHSV vaccine strains and Thailand AHSV-1 field isolate at the 1321–1560 region (numbering according to the OBP AHSV-1 isolate, GenBank accession no. KT030334) by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). OBP strain GenBank accession nos.: AHSV-1, KT030334; AHSV-3, KT030344; AHSV-4, KT030354. Thailand AHSV-1 isolate GenBank accession no.: MT711962. Yellow indicates the primer-binding regions (VP5-DIVA-F/R) and green the probe-binding region (VP5-DIVA-P1). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.
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