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Volume 28, Number 12—December 2022

Research

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. KohComments to Author , Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)

Main Article

Figure 1

Nucleotide sequence alignment of the VP5 gene for the OBP AHSV vaccine strains and Thailand AHSV-1 field isolate at the 1321–1560 region (numbering according to the OBP AHSV-1 isolate, GenBank accession no. KT030334) by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). OBP strain GenBank accession nos.: AHSV-1, KT030334; AHSV-3, KT030344; AHSV-4, KT030354. Thailand AHSV-1 isolate GenBank accession no.: MT711962. Yellow indicates the primer-binding regions (VP5-DIVA-F/R) and green the probe-binding region (VP5-DIVA-P1). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.

Figure 1. Nucleotide sequence alignment of the VP5 gene for the OBP AHSV vaccine strains and Thailand AHSV-1 field isolate at the 1321–1560 region (numbering according to the OBP AHSV-1 isolate, GenBank accession no. KT030334) by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). OBP strain GenBank accession nos.: AHSV-1, KT030334; AHSV-3, KT030344; AHSV-4, KT030354. Thailand AHSV-1 isolate GenBank accession no.: MT711962. Yellow indicates the primer-binding regions (VP5-DIVA-F/R) and green the probe-binding region (VP5-DIVA-P1). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.

Main Article

Page created: October 28, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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