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Volume 28, Number 12—December 2022

Research

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Yifan Wang, Jasmine Ong, Oi Wing Ng, Tapanut Songkasupa, Eileen Y. KohComments to Author , Jeslyn P.S. Wong, Kanokwan Puangjinda, Charlene Judith Fernandez, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, and Him Hoo Yap
Author affiliations: Animal and Veterinary Service, National Parks Board, Singapore (Y. Wang, J. Ong, O.W. Ng, E.Y. Koh, C.J. Fernandez, T. Huangfu, S.F. Chang, H.H. Yap); National Institute of Animal Health, Bangkok, Thailand (T. Songkasupa, K. Puangjinda); Environmental Health Institute, National Environment Agency, Singapore (J.P.S. Wong, L.C. Ng)

Main Article

Figure 2

Nucleotide sequence alignment of the VP5 gene for the OBP vaccine AHSV-1 strain (GenBank accession no. KT030334), Thailand AHSV-1 field isolate (accession no. MT711962), and AHSV-1 field isolate sequences from earlier studies (accession nos. EU303175, KX987212, AM883169 and KT187161) at the 1321–1559 region (numbering according to the OBP AHSV-1 isolate), by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). Yellow indicates the the forward and reverse primer binding regions and green the probe-binding region (VP5-DIVA-P1 or P2). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.

Figure 2. Nucleotide sequence alignment of the VP5 gene for the OBP vaccine AHSV-1 strain (GenBank accession no. KT030334), Thailand AHSV-1 field isolate (accession no. MT711962), and AHSV-1 field isolate sequences from earlier studies (accession nos. EU303175, KX987212, AM883169 and KT187161) at the 1321–1559 region (numbering according to the OBP AHSV-1 isolate), by multiple sequence alignment tool in Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo). Yellow indicates the the forward and reverse primer binding regions and green the probe-binding region (VP5-DIVA-P1 or P2). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals; OBP, Onderstepoort Biologic Products (https://www.obpvaccines.co.za); VP, viral protein.

Main Article

Page created: October 28, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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