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Volume 28, Number 2—February 2022
Research

Role of Anopheles Mosquitoes in Cache Valley Virus Lineage Displacement, New York, USA

Constentin Dieme1Comments to Author , Kiet A. Ngo1, Shaun Tyler, Joseph G. Maffei, Steven D. Zink, Alan P. Dupuis, Cheri A. Koetzner, Chelsea Shultis, Jessica Stout, Anne F. Payne, P. Bryon Backenson, Lili Kuo, Michael A. Drebot, Alexander T. Ciota, and Laura D. Kramer
Author affiliations: New York State Department of Health, Slingerlands, New York, USA (C. Dieme, K.A. Ngo, J.G. Maffei, S.D. Zink, A.P. Dupuis, C.A. Koetzner, C. Shultis, J. Stout, A.F. Payne, L. Kuo, A.T. Ciota, L.D. Kramer); Public Health Agency of Canada, Winnipeg, Manitoba, Canada (S. Tyler, M.A Drebot); New York State Department of Health, Albany, New York, USA (P.B. Backenson); State University of New York at Albany, Albany (A.T. Ciota, L.D. Kramer)

Main Article

Table 2

Primers used for Sanger sequencing of CVV, New York, USA*

Primer Sequence, 5′ → 3′ Target
CVVM AGTAGTGTGCTACCGATA M segment
CVVMR5
 ACTCCTGCCTGCCAGAGTGC
 1–2239 bp
CVVMF4 AATGCATTCCCAGGAACAAC M segment
CVVMR2
 CCTCTAGAGTCTCATGATTA
 1984–3725 bp
CVVMF6 ATCCCTGCATTAGGTGGAAT M segment
CVVM
 AGTAGTGTGCTACCGATA
 2981–4464 bp
CVVrtL CTGACCATACCCGAGAGGCTAGTAGTGTACTCCT L segment
NLR10
 CTGTTGCTCTTTTTGTCTTGATGTCTGAAG
 1–1717 bp
LF3 GGGGGTATTCTCAGACCAGA L segment
NLR7
 GGATCTAAAACTATAAGCCAAAAATACTT
 1482–3221 bp
NLF6 CTAAAGAAAGATGTAAGTTAAATACAGATG L segment
LR4
 CATCAGTGGGTCATTTAATA
 2984–4722 bp
NLF8 ATATCAATGCGCCATTATACCTTATATC L segment
LR2
 CTGACATAAATTCGAACTTC
 3986–5722 bp
LF11b ACAAATTCGATGCTCTAAAAACAA L segment
CVVrtL
 CTGACCATACCCGAGAGGCTAGTAGTGTACTCCT
 5474–6871 bp
CVVrtALL CTGACCATACCCGAGAGGCTAGTGTGTACT S segment
CVVs AGTAGTGTGCTCCAC 1–922 bp

*CVV, Cache Valley virus; L, large; M, medium; S, small.

Main Article

1These authors contributed equally to this article.

Page created: October 17, 2021
Page updated: January 22, 2022
Page reviewed: January 22, 2022
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