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Volume 28, Number 2—February 2022
Research

Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

Arun K. DharComments to Author , Roberto Cruz-Flores, Janet Warg, Mary L. Killian, Andrew Orry, Jorge Ramos, Michelle Garfias, and Gregory Lyons
Author affiliations: The University of Arizona, Tucson, Arizona, USA (A.K. Dhar, R. Cruz-Flores, J. Ramos, M. Garfias, G. Lyons); US Department of Agriculture National Veterinary Services Laboratories, Ames, Iowa, USA (J. Warg, M.L. Killian); Molsoft, Inc., San Diego, California, USA (A. Orry)

Main Article

Figure 1

Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Penaeus vannamei shrimp from the United States by conventional PCR, 2019. Agarose gel photographs show 389-bp IHHNV–specific amplicon (left column) and 309-bp IHHNV–specific amplicon (right column). Top row represents case number 19-490, P. vannamei broodstock samples originating in Florida (A and B). Middle row represents case number 19-428, P. vannamei post-larvae samples originating in Texas (C1 and C2). Bottom row represents case number 19-644, frozen P. vannamei shrimp originating from an indoor farm in Texas (A, B, C, and D). Lane M, 100-bp molecular weight marker (New England Biolabs, Inc., https://www.neb.com); lane SPF, specific pathogen-free P. vannamei shrimp; lane NT, no template control; lane +C, positive control for PCR.

Figure 1. Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Penaeus vannamei shrimp from the United States by conventional PCR, 2019. Agarose gel photographs show 389-bp IHHNV–specific amplicon (left column) and 309-bp IHHNV–specific amplicon (right column). Top row represents case number 19-490, P. vannamei broodstock samples originating in Florida (A and B). Middle row represents case number 19-428, P. vannamei post-larvae samples originating in Texas (C1 and C2). Bottom row represents case number 19-644, frozen P. vannamei shrimp originating from an indoor farm in Texas (A, B, C, and D). Lane M, 100-bp molecular weight marker (New England Biolabs, Inc., https://www.neb.com); lane SPF, specific pathogen-free P. vannamei shrimp; lane NT, no template control; lane +C, positive control for PCR.

Main Article

Page created: December 08, 2021
Page updated: January 24, 2022
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