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Volume 28, Number 2—February 2022
Research

Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

Arun K. DharComments to Author , Roberto Cruz-Flores, Janet Warg, Mary L. Killian, Andrew Orry, Jorge Ramos, Michelle Garfias, and Gregory Lyons
Author affiliations: The University of Arizona, Tucson, Arizona, USA (A.K. Dhar, R. Cruz-Flores, J. Ramos, M. Garfias, G. Lyons); US Department of Agriculture National Veterinary Services Laboratories, Ames, Iowa, USA (J. Warg, M.L. Killian); Molsoft, Inc., San Diego, California, USA (A. Orry)

Main Article

Table 1

Primers used for the detection of the IHHNV genome in commercially raised Penaeus vannamei shrimp, Texas and Florida, United States, 2019*

Target gene Primer name Sequence, 5′ → 3′ Amplicon size, bp Reference
NS1 389F CGGAACACAACCCGACTTTA 389 (13)
NS1 389R GGCCAAGACCAAAATACGAA
NS1 IHHNV-309F TCCAACACTTAGTCAAAACCAA 309 (13)
NS1 IHHNV-309R TGTCTGCTACGATGATTATCCA
NS1 (Left) 159_IH_NS1_S AACTGACGAGTGAAGAGGCT 1295 This study
NS1 (Left) 1446_IH_NS1_AS GTGTCCGGAGTATGTGATGT This study
NS1 (Right) 1400_IH_NS1_S GGACGAACGCCAAACTTCAC 1313 This study
NS1 (Right) 2698_IH_NS1_AS ATCTGTGTGGGTCTGGTCC This study
CP CP1F GATCACCAGCACGACTTCCT 1088 (21)
CP CP2R CGGGTATATATTGCACATCGAA

*CP, capsid protein gene; IHHNV, infectious hypodermal and hematopoietic necrosis virus; NS1, nonstructural gene 1.

Main Article

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Page created: December 08, 2021
Page updated: January 24, 2022
Page reviewed: January 24, 2022
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