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Volume 28, Number 2—February 2022
Research

Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

Arun K. DharComments to Author , Roberto Cruz-Flores, Janet Warg, Mary L. Killian, Andrew Orry, Jorge Ramos, Michelle Garfias, and Gregory Lyons
Author affiliations: The University of Arizona, Tucson, Arizona, USA (A.K. Dhar, R. Cruz-Flores, J. Ramos, M. Garfias, G. Lyons); US Department of Agriculture National Veterinary Services Laboratories, Ames, Iowa, USA (J. Warg, M.L. Killian); Molsoft, Inc., San Diego, California, USA (A. Orry)

Main Article

Table 2

Additional primers designed and used for Sanger sequencing of CP and NS1 genes of IHHNV, Texas and Florida, United States, 2019*

Target gene Primer name Sequence, 5′ → 3′
NS1 159_IH_NS1_S AAC TGA CGA GTG AAG AGG CT
NS1 1446_IH_NS1_AS GTG TCC GGA GTA TGT GAT GT
NS1 482_IHHNV_NS1_S CCC CAA CAA ATA TCG CTG CG
NS1 511_IHHNV_NS1_S AGA TCA CAT TCT ACC GTG GTG
NS1 342_IH_NS1_Seq_AS ACTT GTA CTT ACA TTT GTA T
NS1 1400_IH_NS1_S GGA CGA ACG CCA AAC TTC AC
NS1 2627_IH_NS1_Seq_S CAA GCC CAA GGA AAA GAT CC
NS1 2390 CP_S CTA CTG GGT ACC ACC AGC
NS1 2698 IH_NS1_AS ATC TGT GTG GGT CTG GTC C
NS1 2540 IH_CP_S AGG CCT CTT CCA AGA ATA CG
NS1 2682 IHHNV_NS1_AS ACT TGA TCC TTC GGC GTG TT
NS1 2540 IH_CP_S AGG CCT CTT CCA AGA ATA CG
NS1 2682 IHHNV_NS1_AS ACT TGA TCC TTC GGC GTG TT
NS1 IHHNV-1942_F GTC ACT AAT TAC AAA CCT GCA G
NS1 IHHNV-2020R GCA TAT TGT CGT AGT CTG GT
CP IHHNV-CP_S ATG TGC GCC GAT TCA ACA AG
CP IH_CP_Seq_S CAT AAT CAA CTA TCA ACT AA
CP IH_CP_Seq_AS TGC CAA TGT TAC GTC GGT TTC C

*CP, capsid protein gene; IHHNV, infectious hypodermal and hematopoietic necrosis virus; NS1, nonstructural gene 1.

Main Article

Page created: December 08, 2021
Page updated: January 24, 2022
Page reviewed: January 24, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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