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Volume 28, Number 7—July 2022
Dispatch

Anncaliia algerae Microsporidiosis Diagnosed by Metagenomic Next-Generation Sequencing, China

Chen Liu1, Qin Chen1, Ping Fu, and Yun-Ying ShiComments to Author 
Author affiliations: West China Hospital of Sichuan University, Chengdu, China (C. Liu, Q. Chen, P. Fu, Y.-Y. Shi); Chengdu 363 Hospital of Southwest Medical University, Chengdu (Q. Chen)

Main Article

Figure 2

Light microscopy and transmission electron microscopy of left biceps branchii muscle biopsy tissue from a 45-year-old man with microsporidiosis caused by Anncaliia algerae, China. A–D) Light microscopy using different stains. A) Periodic acid-Schiff stain. Scale bar indicates 10 µm. Original magnification ×50. B) Gomori methenamine silver stain. Scale bar indicates 10 µm. Original magnification ×63. C) Warthin-Starry stain. Scale bar indicates 10 µm. Original magnification ×63. D) Toluidine blue stain. Scale bar indicates 10 µm. Arrows indicate myocytes replaced by aggregates of 2–3 µm ovoid organisms. Original magnification ×63. E, F) Transmission electron microscopy showing Anncaliia-like microsporidia. Scale bars indicate 500 nm. E) A mature spore with electron-dense exospore, electron-lucent endospore, and a single row of 6 to 8 polar tubule coils (arrows). Original magnification ×8,000. F) Proliferating form of microsporidia showing diplokaryotic nuclei (stars) with vesiculotubular structures extending from the meront cell membrane and aggregating in the host cell cytoplasm (arrows). Original magnification ×3,000.

Figure 2. Light microscopy and transmission electron microscopy of left biceps branchii muscle biopsy tissue from a 45-year-old man with microsporidiosis caused by Anncaliia algerae, China. A–D) Light microscopy using different stains. A) Periodic acid-Schiff stain. Scale bar indicates 10 µm. Original magnification ×50. B) Gomori methenamine silver stain. Scale bar indicates 10 µm. Original magnification ×63. C) Warthin-Starry stain. Scale bar indicates 10 µm. Original magnification ×63. D) Toluidine blue stain. Scale bar indicates 10 µm. Arrows indicate myocytes replaced by aggregates of 2–3 µm ovoid organisms. Original magnification ×63. E, F) Transmission electron microscopy showing Anncaliia-like microsporidia. Scale bars indicate 500 nm. E) A mature spore with electron-dense exospore, electron-lucent endospore, and a single row of 6 to 8 polar tubule coils (arrows). Original magnification ×8,000. F) Proliferating form of microsporidia showing diplokaryotic nuclei (stars) with vesiculotubular structures extending from the meront cell membrane and aggregating in the host cell cytoplasm (arrows). Original magnification ×3,000.

Main Article

1These first authors contributed equally to this article.

Page created: May 16, 2022
Page updated: June 18, 2022
Page reviewed: June 18, 2022
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