Figure 4. Annotated plasmid maps of the dominant carbapenemase-encoding plasmid in clinical Enterobacterales isolates and hypervirulent Klebsiella pneumoniae, Singapore. A) Annotated plasmid map of pKPC2_sg1 (GenBank accession no. MN542377), including the complete conjugative machinery (oriT, relaxase, T4CP, and T4SS) and the resistance genes, blaKPC-2, blaTEM-1, mph(A), and TEM family class A β-lactamase (TEM-1). The region of pKPC2_sg1 encoding the resistance genes was found in another plasmid called pKPCAPSS (GenBank accession no. KP008371), but the region encoding the conjugative machinery was highly similar to the sequence of pSA20021456.2 (GenBank accession no. CP030221). B) Plasmid alignment map showing other environmental or clinical plasmids with similar backbone, the pSA20021456.2 backbone, as pKPC2. C) Graphical representation of EcoRI/BamHI digested pKPC2 region containing replicon.