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Volume 30, Number 8—August 2024
Research

SARS-CoV-2 Seropositivity in Urban Population of Wild Fallow Deer, Dublin, Ireland, 2020–2022

Kevin Purves1, Hannah Brown1, Ruth Haverty, Andrew Ryan, Laura L. Griffin, Janet McCormack, Sophie O’Reilly, Patrick W. Mallon, Virginie Gautier, Joseph P. Cassidy, Aurelie Fabre, Michael J. Carr, Gabriel Gonzalez, Simone Ciuti, and Nicola F. FletcherComments to Author 
Author affiliations: University College Dublin, Dublin, Ireland (K. Purves, H. Brown, R. Haverty, A. Ryan, L.L. Griffin, J. McCormack, S. O’Reilly, P.W. Mallon, V. Gautier, J.P. Cassidy, A. Fabre, M.J. Carr, G. Gonzalez, S. Ciuti, N.F. Fletcher); St Vincent's University Hospital, Dublin (P.W. Mallon, A. Fabre); Hokkaido University, Sapporo, Japan (M.J. Carr, G. Gonzalez)

Main Article

Figure 2

Infectivity of SARS-CoV-2 pseudoviruses after incubation with SARS-CoV-2–positive serum samples from wild fallow deer, Dublin, Ireland, 2020–2022. Spike proteins were from Alpha (A), Delta (B), Omicron BA.1 (C), and Omicron BA.2 (D) variants of concern. SARS-CoV-2 pseudoviruses bearing spike proteins from different variants of concern were incubated with 5 deer serum samples at a 1:1 ratio in triplicate and then used to infect Vero E6/TMPRSS2 cells. Identification numbers of deer are indicated. Controls were virus incubated in triplicate at a 1:1 ratio with culture medium. Relative light units from a luciferase reporter were used to calculate percentage infectivity relative to the untreated control virus. Data are from 2 independent experiments with 3 biologic replicates per experiment. Error bars indicate SDs. NE, no envelope naked pseudovirus control; NS, not significant; UF, uninfected cells.

Figure 2. Infectivity of SARS-CoV-2 pseudoviruses after incubation with SARS-CoV-2–positive serum samples from wild fallow deer, Dublin, Ireland, 2020–2022. Spike proteins were from Alpha (A), Delta (B), Omicron BA.1 (C), and Omicron BA.2 (D) variants of concern. SARS-CoV-2 pseudoviruses bearing spike proteins from different variants of concern were incubated with 5 deer serum samples at a 1:1 ratio in triplicate and then used to infect Vero E6/TMPRSS2 cells. Identification numbers of deer are indicated. Controls were virus incubated in triplicate at a 1:1 ratio with culture medium. Relative light units from a luciferase reporter were used to calculate percentage infectivity relative to the untreated control virus. Data are from 2 independent experiments with 3 biologic replicates per experiment. Error bars indicate SDs. NE, no envelope naked pseudovirus control; NS, not significant; UF, uninfected cells.

Main Article

1These authors contributed equally to this article.

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