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Volume 30, Number 8—August 2024
Research

SARS-CoV-2 Seropositivity in Urban Population of Wild Fallow Deer, Dublin, Ireland, 2020–2022

Kevin Purves1, Hannah Brown1, Ruth Haverty, Andrew Ryan, Laura L. Griffin, Janet McCormack, Sophie O’Reilly, Patrick W. Mallon, Virginie Gautier, Joseph P. Cassidy, Aurelie Fabre, Michael J. Carr, Gabriel Gonzalez, Simone Ciuti, and Nicola F. FletcherComments to Author 
Author affiliations: University College Dublin, Dublin, Ireland (K. Purves, H. Brown, R. Haverty, A. Ryan, L.L. Griffin, J. McCormack, S. O’Reilly, P.W. Mallon, V. Gautier, J.P. Cassidy, A. Fabre, M.J. Carr, G. Gonzalez, S. Ciuti, N.F. Fletcher); St Vincent's University Hospital, Dublin (P.W. Mallon, A. Fabre); Hokkaido University, Sapporo, Japan (M.J. Carr, G. Gonzalez)

Main Article

Figure 3

Infectivity of SARS-CoV-2 infectious viruses after incubation with SARS-CoV-2–positive serum samples from wild fallow deer, Dublin, Ireland, 2020–2022. Deer serum samples were incubated with infectious SARS-CoV-2 ancestral strain Italy_INMI1 (A) or Omicron BA.1 (B) and then used to infect Vero E6/TMPRSS2 cells. Identification numbers of deer are indicated. Cytopathic effect was calculated as TCID50, as previously described (19). Data are from 2 independent experiments with 8 biologic replicates per experiment. p values were calculated by using 1-way analysis of variance (Appendix 2 Tables 2, 3). Error bars indicate SDs. NS, not significant; TCID50, 50% tissue culture infectious dose.

Figure 3. Infectivity of SARS-CoV-2 infectious viruses after incubation with SARS-CoV-2–positive serum samples from wild fallow deer, Dublin, Ireland, 2020–2022. Deer serum samples were incubated with infectious SARS-CoV-2 ancestral strain Italy_INMI1 (A) or Omicron BA.1 (B) and then used to infect Vero E6/TMPRSS2 cells. Identification numbers of deer are indicated. Cytopathic effect was calculated as TCID50, as previously described (19). Data are from 2 independent experiments with 8 biologic replicates per experiment. p values were calculated by using 1-way analysis of variance (Appendix 2 Tables 2, 3). Error bars indicate SDs. NS, not significant; TCID50, 50% tissue culture infectious dose.

Main Article

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1These authors contributed equally to this article.

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