Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 30, Number 9—September 2024
Research

Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes

Cristina Rafferty, Gloria Raise, JeNyiah Scaife, Bernard Abongo, Seline Omondi, Sylvia Milanoi, Margaret Muchoki, Brenda Onyango, Eric Ochomo, and Sarah ZohdyComments to Author 
Author affiliations: US President’s Malaria Initiative, Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Rafferty, S. Zohdy); Centers for Disease Control and Prevention, Atlanta (C. Rafferty, G. Raise, J. Scaife, S. Zohdy); Kenya Medical Research Institute (KEMRI), Kisumu, Kenya (B. Abongo, S. Omondi, S. Milanoi, M. Muchoki, B. Onyango, E. Ochomo)

Main Article

Figure 3

Schematic for colorimetric loop-mediated isothermal amplification assay to detect invasive malaria vector Anopheles stephensi mosquitoes. Top: DNA from any mosquito source directly placed in the colorimetric master mix are incubated at 65°C for 30 minutes to obtain a yellow color change, showing a positive sample. The assay shows high sensitivity and specificity when DNA extract from adult or larval mosquitoes is used. Bottom: Assay can also be used directly on a single mosquito leg, without the need for DNA extraction, by adding a 5-minute extension to incubation time (i.e., 35 minutes). Schematic produced using Biorender (https://www.biorender.com).

Figure 3. Schematic for colorimetric loop-mediated isothermal amplification assay to detect invasive malaria vector Anopheles stephensi mosquitoes. Top: DNA from any mosquito source directly placed in the colorimetric master mix are incubated at 65°C for 30 minutes to obtain a yellow color change, showing a positive sample. The assay shows high sensitivity and specificity when DNA extract from adult or larval mosquitoes is used. Bottom: Assay can also be used directly on a single mosquito leg, without the need for DNA extraction, by adding a 5-minute extension to incubation time (i.e., 35 minutes). Schematic produced using Biorender (https://www.biorender.com).

Main Article

Page created: July 10, 2024
Page updated: August 20, 2024
Page reviewed: August 20, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external