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Volume 30, Number 9—September 2024
Research

Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes

Cristina Rafferty, Gloria Raise, JeNyiah Scaife, Bernard Abongo, Seline Omondi, Sylvia Milanoi, Margaret Muchoki, Brenda Onyango, Eric Ochomo, and Sarah ZohdyComments to Author 
Author affiliations: US President’s Malaria Initiative, Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Rafferty, S. Zohdy); Centers for Disease Control and Prevention, Atlanta (C. Rafferty, G. Raise, J. Scaife, S. Zohdy); Kenya Medical Research Institute (KEMRI), Kisumu, Kenya (B. Abongo, S. Omondi, S. Milanoi, M. Muchoki, B. Onyango, E. Ochomo)

Main Article

Table 1

Looped primers designed by using the NEB LAMP Primer Design Tool targeting Anopheles stephensi mosquito ITS2 sequence regions for development of colorimetric LAMP assay to detect invasive malaria vector An. stephensi mosquitoes*

Sequence 5′ 3′ Primer sequence Primer concentration
F3 333 351 ATTGCACGGGGACTTCCA 5 µM
B3 504 524 GCCTACAGACTCCACTGTCA 5 µM
FIP CGACTGCAACTGTATGCGAGGACGGGTCGAGTAACACTTGC 20 µM
BIP CCGTGTGGGTGAGTGAGGTTAGAATGATGCGACGGGAGAAG 20 µM
LF 383 401 AAGATACGAGCGCGTTGGG 10 µM
F1c† 402 423 CGACTGCAACTGTATGCG
F2† 359 380 GGACGGGTCGAGTAACACTTGC
B2† 439 461 CCGTGTGGGTGAGTGAGGTTAG
B1c† 485 502 AATGATGCGACGGGAGAAG

*ITS, internal transcribed spacer; LAMP, loop-mediated isothermal amplification; NEB, New England Biolabs, https://www.neb.com. †Primers not needed for LAMP assay.

Main Article

Page created: July 10, 2024
Page updated: August 20, 2024
Page reviewed: August 20, 2024
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