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Volume 30, Number 9—September 2024
Research
Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes
Cristina Rafferty, Gloria Raise, JeNyiah Scaife, Bernard Abongo, Seline Omondi, Sylvia Milanoi, Margaret Muchoki, Brenda Onyango, Eric Ochomo, and Sarah Zohdy
Table 1
Looped primers designed by using the NEB LAMP Primer Design Tool targeting Anopheles stephensi mosquito ITS2 sequence regions for development of colorimetric LAMP assay to detect invasive malaria vector An. stephensi mosquitoes*
| Sequence | 5′ | 3′ | Primer sequence | Primer concentration |
|---|---|---|---|---|
| F3 | 333 | 351 | ATTGCACGGGGACTTCCA | 5 µM |
| B3 | 504 | 524 | GCCTACAGACTCCACTGTCA | 5 µM |
| FIP | CGACTGCAACTGTATGCGAGGACGGGTCGAGTAACACTTGC | 20 µM | ||
| BIP | CCGTGTGGGTGAGTGAGGTTAGAATGATGCGACGGGAGAAG | 20 µM | ||
| LF | 383 | 401 | AAGATACGAGCGCGTTGGG | 10 µM |
| F1c† | 402 | 423 | CGACTGCAACTGTATGCG | |
| F2† | 359 | 380 | GGACGGGTCGAGTAACACTTGC | |
| B2† | 439 | 461 | CCGTGTGGGTGAGTGAGGTTAG | |
| B1c† | 485 | 502 | AATGATGCGACGGGAGAAG |
*ITS, internal transcribed spacer; LAMP, loop-mediated isothermal amplification; NEB, New England Biolabs, https://www.neb.com. †Primers not needed for LAMP assay.
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