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Volume 30, Number 9—September 2024
Research
Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes
Table 3
Primer set candidates tested with different concentrations to determine effects on sensitivity and specificity of colorimetric loop-mediated isothermal amplification assay to detect invasive malaria vector Anopheles stephensi mosquitoes
Concentration combination | Primer set* |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Primer concentration |
P26L2 |
P2L-45 |
|||||||||
F3 | B3 | FIP | B IP | Loop primer | Sensitivity | Specificity | Sensitivity | Specificity | |||
A1 | 2 µM | 2 µM | 16 µM | 16 µM | 4 µM | X | ✓ | X | X | ||
A2 | 4 µM | 4 µM | 16 µM | 16 µM | 4 µM | ✓ | X | X | X | ||
A3 | 2 µM | 2 µM | 32 µM | 32 µM | 4 µM | ✓ | X | X | X | ||
A4 | 2 µM | 2 µM | 16 µM | 16 µM | 8 µM | X | ✓ | X | X | ||
B1 | 5 µM | 5 µM | 40 µM | 40 µM | 10 µM | ✓ | X | ✓ | X | ||
B2 | 2.5 µM | 2.5 µM | 40 µM | 40 µM | 10 µM | ✓ | X | X | ✓ | ||
B3 | 5 µM | 5 µM | 20 µM | 20 µM | 10 µM | ✓ | ✓ | X | X | ||
B4 | 5 µM | 5 µM | 40 µM | 40 µM | 5 µM | ✓ | X | X | X |
*For each primer set, a check mark indicates consistent results and an X inconsistent results.
Page created: July 10, 2024
Page updated: July 10, 2024
Page reviewed: July 10, 2024
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