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Volume 30, Number 9—September 2024
Research
Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes
Cristina Rafferty, Gloria Raise, JeNyiah Scaife, Bernard Abongo, Seline Omondi, Sylvia Milanoi, Margaret Muchoki, Brenda Onyango, Eric Ochomo, and Sarah Zohdy
Table 5
Specificity testing for colorimetric loop-mediated isothermal amplification assay to detect invasive malaria vector Anopheles stephensi mosquitoes*
| Species | Colony name | No. mosquitoes | Experiment 1 | Experiment 2 | Experiment 3 |
|---|---|---|---|---|---|
| An. stephensi | STE2 | 96 | Positive (96/96) | Positive (96/96) | Positive (96/96) |
| An. stephensi | SDA500 | 96 | Positive (96/96) | Positive (96/96) | Positive (96/96) |
| An. stephensi | UCI | 96 | Positive (96/96) | Positive (96/96) | Positive (96/96) |
| An. gambiae s.s | G3 | 48 | Negative (0/48) | Negative (0/48) | Negative (0/48) |
| An. coluzzii | AKDR | 48 | Negative (0/48) | Negative (0/48) | Negative (0/48) |
| An. arabiensis | DONGOLA | 48 | Negative (0/48) | Negative (0/48) | Negative (0/48) |
| An. funestus s.s | FANG | 48 | Negative (0/48) | Negative (0/48) | Negative (0/48) |
| Aedes aegypti |
ROCK |
48 |
Negative (0/48) |
Negative (0/48) |
Negative (0/48) |
| Total no. samples |
528 |
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| Total no. reactions | 1,584 |
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*To determine specificity, we ran the essay in triplicate on extracted DNA from 96 individual mosquitoes from each of the 3 An. stephensi insectary strains and DNA extracted from 48 individual mosquitoes from each species of An. gambiae sensu stricto, An. coluzzii, An. arabiensis, An. funestus, and Ae. aegypti.
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