Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Volume 31, Number 11—November 2025

Research

Isolation and Characterization of Rickettsia finnyi, Novel Pathogenic Spotted Fever Group Rickettsia in Dogs, United States

Praveen K. Korla1, Michael G. Karounos1, Sarah B. Clarke, Cynthia Robveille, James M. Wilson, Edward B. Breitschwerdt, Adam J. Birkenheuer, and Barbara A. QurolloComments to Author 
Author affiliation: North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina, USA

Main Article

Table 1

Growth of Rickettsia finnyi sp. nov. strain 2024-CO-Wats in Vero E6, 030D, and DH82 cells monitored through changing Cq values in Rickettsia 23s-5s ITS and R. finnyi–specific M61 qPCRs in study of isolation and characterization of novel pathogenic spotted fever group Rickettsia in dogs, United States*

Day Vero E6
 030D
 DH82
Sample Rickettsia 23S-5S (Cq) R. finnyi M61 probe (Cq) Sample Rickettsia 23S-5S (Cq) R. finnyi M61 probe (Cq) Sample Rickettsia 23S-5S (Cq) R. finnyi M61 probe (Cq)
3 VE6–5† 28.88 NA 030D-3† 33.32 NA DH82–3† 31.35 NA
6 VE6–5† 28.88 NA 030D-3-P1† 35.13 NA DH82–3-P1† 33.38 NA
10 VE6–5† 28.41 32.26 030D-3-P1† 30.37 34.52 DH82–3-P1† 32.83 35.76
13 VE6–5-P1† 30.40 34.28 030D-3 -P1† 30.86 34.45 DH82–3-P1† 28.93 32.60
20 VE6–5-P2† 21.41 25.88 030D-3-P2† 25.27 29.48 DH82–3-P2† 24.74 29.31
32 VE6–5-P4†‡ 16.06 17.20 030D-3-P4† 20.35 21.67 DH82–3-P4† 19.49 22.78
57 NA NA NA NA NA NA DH82–3-P7† 18.70 NA
89 VE6–5-P7 12.81 NA NA NA NA NA NA NA
104 VE6–5-P9†‡ 15.73 NA 030D-3-P15† 18.59 NA DH82–3-P14† 18.93 NA

*Cq, quantification cycle; NA, not applicable; qPCR, quantitative PCR.
†qPCR performed to identify an MFS transporter single nucleotide mutation T→G.
‡MFS transporter single nucleotide mutation A→C present

Main Article

1These authors contributed equally to this article.

Page created: September 24, 2025
Page updated: November 26, 2025
Page reviewed: November 26, 2025
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external