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Volume 31, Number 11—November 2025

Research

Two Independent Acquisitions of Multidrug Resistance Gene lsaC in Streptococcus pneumoniae Serotype 20 Multilocus Sequence Type 1257

Bernard BeallComments to Author , Wuling Lin, Zhongya Li, Theresa Tran, Benjamin J. Metcalf, Bridget J. Anderson, Keipp H. Talbot, Lesley McGee, and Sopio Chochua
Author affiliation: ASRT, Inc., Smyrna, Georgia, USA (B. Beall, Z. Li); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (W. Lin, T. Tran, B.J. Metcalf, L. McGee, S. Chochua); New York State Department of Health, Albany, New York, USA (B.J. Anderson); Vanderbilt University School of Medicine, Nashville, Tennessee, USA (K.H. Talbot)

Main Article

Figure 5

Single contig genome of isolate 20234456 (msrD-positive, mef-positive, tetM-positive, lsaC-positive) representative of isolates 1–16 in study of independent acquisitions of multidrug resistance gene lsaC in serotype 20/ST1257 Streptococcus pneumoniae isolates, United States. Position 1 starts at the dnaA structural gene. CDSs and tRNA genes are indicated on the forward strand (outer band) and reverse strand (inner band). The genes approximating the location of the interspecies double-crossover event introducing the resistance element lie between the rpsD ribosomal protein gene and the putative transport gene orf17. The ribosomal protein gene rplL is also depicted near the 1.2-Mbp marker to show the approximate genomic location of the accessory element carrying the ermB, tetM, and lsaC determinants in isolate 17. Other determinants shown reflect potential genomic resistance determinants (all indicative of susceptibility in this genome according to bioinformatic pipeline output), including folP/folA (cotrimoxazole); parC/gyrA (fluoroquinolones); and pbp2x/pbp1a/pbp2b (β-lactam antibiotics). In addition, the multilocus sequence typing markers spi-15, gki-8, aroE-15, ddl-31, xpt-31, and recp-18 define ST1257. CDS, coding sequences; ST, sequence type.

Figure 5. Single contig genome of isolate 20234456 (msrD-positive, mef-positive, tetM-positive, lsaC-positive) representative of isolates 1–16 in study of independent acquisitions of multidrug resistance gene lsaC in serotype 20/ST1257 Streptococcus pneumoniae isolates, United States. Position 1 starts at the dnaA structural gene. CDSs and tRNA genes are indicated on the forward strand (outer band) and reverse strand (inner band). The genes approximating the location of the interspecies double-crossover event introducing the resistance element lie between the rpsD ribosomal protein gene and the putative transport gene orf17. The ribosomal protein gene rplL is also depicted near the 1.2-Mbp marker to show the approximate genomic location of the accessory element carrying the ermB, tetM, and lsaC determinants in isolate 17. Other determinants shown reflect potential genomic resistance determinants (all indicative of susceptibility in this genome according to bioinformatic pipeline output), including folP/folA (cotrimoxazole); parC/gyrA (fluoroquinolones); and pbp2x/pbp1a/pbp2b (β-lactam antibiotics). In addition, the multilocus sequence typing markers spi-15, gki-8, aroE-15, ddl-31, xpt-31, and recp-18 define ST1257. CDS, coding sequences; ST, sequence type.

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Page created: September 19, 2025
Page updated: November 25, 2025
Page reviewed: November 25, 2025
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