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Volume 31, Supplement—May 2025
SUPPLEMENT ISSUE
Supplement
16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023
Guyu Li
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
Table 1
Patient characteristics from study of 16S ribosomal RNA gene PCR and sequencing for pediatric infection diagnosis, Mayo Clinic, Rochester, Minnesota, USA, 2020–2023*
| Patient characteristics | Value, n = 124 patients |
|---|---|
| Median age, y (IQR) |
9.6 (2.2–15.0) |
| Sex | |
| F | 58 (47) |
| M |
66 (53) |
| Immunocompromised host† |
24 (20) |
| Intensive care unit admission |
46 (37) |
| Suspected clinical syndrome | |
| Meningoencephalitis | 38 (31) |
| Musculoskeletal infection: septic arthritis, osteomyelitis | 33 (27) |
| Pleural effusion | 10 (8) |
| Surgical wound infection, including hardware infection | 10 (8) |
| Lymphadenopathy | 5 (4) |
| Bone mass | 4 (3) |
| Intracranial abscess/fluid collection | 2 (2) |
| Pericardial effusion | 4 (3) |
| Pulmonary nodules | 4 (3) |
| Traumatic wound infection | 4 (3) |
| Intraabdominal abscess/fluid collection | 4 (3) |
| Endocarditis | 2 (2) |
| Splenic mass | 1 (1) |
| Infected pseudoaneurysms | 1 (1) |
| Mediastinitis | 1 (1) |
| Retinal detachment | 1 (1) |
| Suggested by pediatric infectious diseases team | 56 (45) |
*Values are no. (%) patients except as indicated. IQR, interquartile range. †Immunocompromised hosts include those with history of solid organ transplant, history of hematopoietic stem cell transplant, active chemotherapy, or receiving an immunosuppressive agent.
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