Volume 31, Supplement—May 2025
SUPPLEMENT ISSUE
Supplement
16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023
Guyu Li
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
Table 3
Microorganisms detected by 16S rRNA PCR and sequencing and associated specimen sources from a study of 16S ribosomal RNA gene PCR and sequencing for pediatric infection diagnosis, Mayo Clinic, Rochester, Minnesota, USA, 2020–2023
| Identified bacteria | No. (%) positive results, n = 33 | Specimen source (no. tests) |
|---|---|---|
| Polymicrobial | 12 (36) | Bone tissue (4*), subdural fluid (3*), cerebrospinal fluid (1), peri-implant tissue (1), vitreous fluid (1*), synovial tissue (1), peritoneal fluid (1*) |
| Staphylococcus aureus complex | 4 (12) | Synovial fluid (4) |
| Kingella kingae | 3 (9) | Synovial fluid (3*) |
| Streptococcus mitis group | 3 (9) | Pleural fluid (1), pleural fluid (2*) |
| Fusobacterium naviforme/nucleatum | 2 (6) | Synovial fluid (1*), CSF (1*) |
| Streptococcus intermedius | 2 (6) | Cerebrospinal fluid (2) |
| Cardiobacterium hominis | 1 (3) | Peri-implant tissue (1*) |
| Enterococcus faecalis | 1 (3) | Heart valve tissue (1) |
| Fusobacterium necrophorum | 1 (3) | Pleural fluid (1) |
| Pseudomonas aeruginosa | 1 (3) | Synovial tissue (1*) |
| Staphylococcus epidermidis | 1 (3) | Cerebrospinal fluid (1) |
| Streptococcus dysgalactiae | 1 (3) | Synovial fluid (1*) |
| Streptococcus pyogenes | 1 (3) | Pleural fluid (1*) |
*Specimens with negative bacterial cultures from the same specimen source.
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