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Volume 31, Supplement—May 2025
SUPPLEMENT ISSUE
Supplement
16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023
Guyu Li
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
Table 4
Comparison of conventional tests and 16S rRNA PCR and sequencing results from study of 16S ribosomal RNA gene PCR and sequencing for pediatric infection diagnosis, Mayo Clinic, Rochester, Minnesota, USA, 2020–2023*
| Conventional tests characteristics | 16S rRNA PCR and sequencing results |
p value | |
|---|---|---|---|
| Positive | Negative | ||
| Gram stain | |||
| Positive | 7 | 0 | <0.0001 |
| Negative |
26 |
119 |
|
| Bacterial culture | |||
| Positive | 14 | 9 | <0.0001 |
| Negative |
19 |
119 |
|
| BioFire Meningitis/Encephalitis panel, cerebrospinal fluid only | |||
| Positive | 0 | 2† | 1.000 |
| Negative |
2 |
19 |
|
| Synovial fluid Kingella kingae PCR, when clinically ordered | |||
| Positive | 3 | 0 | 0.143 |
| Negative | 1 | 3 | |
*Specimens were collected from same specimen source. †One panel was positive for parechovirus and another was positive for human herpesvirus 6.
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