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Volume 31, Supplement—May 2025
SUPPLEMENT ISSUE
Supplement

16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023

Guyu LiComments to Author , Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
Author affiliation: Mayo Clinic, Rochester, Minnesota, USA (G. Li, C.A. Reis, R.M. Kruc, N.T. Streck, E.H. Ristagno, J. Mandrekar, M.J. Wolf, J.T. Gaensbauer, R. Patel); Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA (Z. Zhang)

Main Article

Table 5

Characteristics of multiple 16S rRNA PCR and sequencing tests ordered on the same patient from the same specimen source during the same procedure from study of 16S ribosomal RNA gene PCR and sequencing for pediatric infection diagnosis, Mayo Clinic, Rochester, Minnesota, USA, 2020–2023*

Characteristics Bone tissue, n = 7 Deep soft tissue, n = 6 Synovial fluid, n = 4 Cerebrospinal fluid, n = 2 Brain tissue, n = 1 Pacemaker pocket tissue, n = 1 Subdural fluid, n = 1
Frequency of tests ordered
Two 4 (57) 3 (50) 3 (75) 2 (100) 1 (100) 1 (100) 0
Three 2 (29) 3 (50) 1 (25) 0 0 0 1 (100)
Four
 1 (14)
 0
 0
 0
 0
 0
 0
Assay results
Concordant negative 6 (86) 6 (100) 3 (75) 1 (50) 1 (100) 1 (100) 0
Concordant positive 1 (14) 0 1 (25) 1 (50) 0 0 1 (100)
Discordant 0 0 0 0 0 0 0

*Values are no. (%) patients.

Main Article

Page created: April 11, 2025
Page updated: May 08, 2025
Page reviewed: May 08, 2025
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