Volume 31, Supplement—May 2025
SUPPLEMENT ISSUE
Supplement
16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023
Guyu Li
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
, Christopher A. Reis, Rebecca M. Kruc, Ziyuan Zhang, Nicholas T. Streck, Elizabeth H. Ristagno, Jay Mandrekar, Matthew J. Wolf, James T. Gaensbauer, and Robin Patel
Table 5
Characteristics of multiple 16S rRNA PCR and sequencing tests ordered on the same patient from the same specimen source during the same procedure from study of 16S ribosomal RNA gene PCR and sequencing for pediatric infection diagnosis, Mayo Clinic, Rochester, Minnesota, USA, 2020–2023*
| Characteristics | Bone tissue, n = 7 | Deep soft tissue, n = 6 | Synovial fluid, n = 4 | Cerebrospinal fluid, n = 2 | Brain tissue, n = 1 | Pacemaker pocket tissue, n = 1 | Subdural fluid, n = 1 |
|---|---|---|---|---|---|---|---|
| Frequency of tests ordered | |||||||
| Two | 4 (57) | 3 (50) | 3 (75) | 2 (100) | 1 (100) | 1 (100) | 0 |
| Three | 2 (29) | 3 (50) | 1 (25) | 0 | 0 | 0 | 1 (100) |
| Four |
1 (14) |
0 |
0 |
0 |
0 |
0 |
0 |
| Assay results | |||||||
| Concordant negative | 6 (86) | 6 (100) | 3 (75) | 1 (50) | 1 (100) | 1 (100) | 0 |
| Concordant positive | 1 (14) | 0 | 1 (25) | 1 (50) | 0 | 0 | 1 (100) |
| Discordant | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
*Values are no. (%) patients.
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