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Volume 31, Number 2—February 2025
Research

Cyclospora Genotypic Variations and Associated Epidemiologic Characteristics, United States, 2018–2021

John Shen, Vitaliano A. Cama1, David Jacobson, Joel Barratt, and Anne Straily1Comments to Author 
Author affiliation: Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (J. Shen); Centers for Disease Control and Prevention, Atlanta, Georgia, USA. (J. Shen, V.A. Cama, D. Jacobson, J. Barratt, A. Straily)

Main Article

Figure 1

Schematic representation of a cluster consensus genotype in study of Cyclospora genotypic variations and associated epidemiologic characteristics United States, 2018–2021. Genotypes are derived from 8 markers, 2 Mt and 6 Nu; this schematic representation is based on 1 Mt and 1 Nu marker, where the haplotypes for this Mt marker are A or B, and the Nu haplotypes are C, D, or E. The Centers for Disease Control and Prevention Cyclospora genotyping system, Cybernetic Clustering Of Nonclonal Eukaryotes (CYCLONE) bioinformatic workflow, was used to determine the genetic similarity and clustered specimens 1, 2, and 3 in genetic cluster 1. Specimens 1 and 3 have genotype ACE, and specimen 2 has genotype ADE. Because genotype ACE is present in ≥50% of samples, it is the CCG for cluster 1, and its short name for this example is S001. The corresponding allele for this specific CCG is MA01 (Mt marker A) NA01 (Nu markers C and E). CCG, cluster consensus genotype; ID, identification; Mt, mitochondrial; Nu, nuclear.

Figure 1. Schematic representation of a cluster consensus genotype in study of Cyclospora genotypic variations and associated epidemiologic characteristics United States, 2018–2021. Genotypes are derived from 8 markers, 2 Mt and 6 Nu; this schematic representation is based on 1 Mt and 1 Nu marker, where the haplotypes for this Mt marker are A or B, and the Nu haplotypes are C, D, or E. The Centers for Disease Control and Prevention Cyclospora genotyping system, Cybernetic Clustering Of Nonclonal Eukaryotes (CYCLONE) bioinformatic workflow, was used to determine the genetic similarity and clustered specimens 1, 2, and 3 in genetic cluster 1. Specimens 1 and 3 have genotype ACE, and specimen 2 has genotype ADE. Because genotype ACE is present in ≥50% of samples, it is the CCG for cluster 1, and its short name for this example is S001. The corresponding allele for this specific CCG is MA01 (Mt marker A) NA01 (Nu markers C and E). CCG, cluster consensus genotype; ID, identification; Mt, mitochondrial; Nu, nuclear.

Main Article

1These senior authors were co–principal investigators.

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