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Volume 31, Number 4—April 2025
Research

Detection and Decontamination of Chronic Wasting Disease Prions during Venison Processing

Marissa Milstein, Sarah C. Gresch, Marc D. Schwabenlander, Manci Li, Jason C. Bartz, Damani N. Bryant, Peter R. Christenson, Laramie L. Lindsey, Nicole Lurndahl, Sang-Hyun Oh, Gage R. Rowden, Rachel L. Shoemaker, Tiffany M. Wolf, Peter A. Larsen, and Stuart S. LichtenbergComments to Author 
Author affiliation: University of Minnesota, St. Paul, Minnesota, USA (M. Milstein, S.C. Gresch, M.D. Schwabenlander, M. Li, D.N. Bryant, L.L. Lindsey, N. Lurndahl, G.R. Rowden, R.L. Shoemaker, T.M. Wolf, P.A. Larsen, S.S. Lichtenberg); University of Minnesota, Minneapolis, Minnesota, USA (M. Li, P.R. Christenson, S.-H. Oh); Creighton University, Omaha, Nebraska, USA (J.C. Bartz)

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Figure 5

Results of real-time quaking-induced conversion conducted on meat grinder swab samples in study of detection and decontamination of chronic wasting disease prions during venison processing. A) Rate of amyloid formation; B) maxpoint ratio (ratio of the maximum value to the initial reading); C) maximum slope. Results are shown for negative controls (before meat grinder was used; top row) and after homogenate (after chronic wasting disease–positive muscle was passed through; bottom row). Each dot is an average of a single biologic replicate (consisting of 8 technical replicates). Each set of paired samples (e.g., cast iron worm spindles) resulted in a statistically significant difference post homogenate compared with the negative samples (p<0.05; data not shown). Dashed lines indicate the cutoff for significance using the maxpoint ratio (28). PC, plate cutter; SRT, screw ring threads; WS, worm spindle.

Figure 5. Results of real-time quaking-induced conversion conducted on meat grinder swab samples in study of detection and decontamination of chronic wasting disease prions during venison processing. A) Rate of amyloid formation; B) maxpoint ratio (ratio of the maximum value to the initial reading); C) maximum slope. Results are shown for negative controls (before meat grinder was used; top row) and after homogenate (after chronic wasting disease–positive muscle was passed through; bottom row). Each dot is an average of a single biologic replicate (consisting of 8 technical replicates). Each set of paired samples (e.g., cast iron worm spindles) resulted in a statistically significant difference post homogenate compared with the negative samples (p<0.05; data not shown). Dashed lines indicate the cutoff for significance using the maxpoint ratio (28). PC, plate cutter; SRT, screw ring threads; WS, worm spindle.

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Page created: February 07, 2025
Page updated: March 24, 2025
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