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Volume 31, Number 4—April 2025
Research

Detection and Decontamination of Chronic Wasting Disease Prions during Venison Processing

Marissa Milstein, Sarah C. Gresch, Marc D. Schwabenlander, Manci Li, Jason C. Bartz, Damani N. Bryant, Peter R. Christenson, Laramie L. Lindsey, Nicole Lurndahl, Sang-Hyun Oh, Gage R. Rowden, Rachel L. Shoemaker, Tiffany M. Wolf, Peter A. Larsen, and Stuart S. LichtenbergComments to Author 
Author affiliation: University of Minnesota, St. Paul, Minnesota, USA (M. Milstein, S.C. Gresch, M.D. Schwabenlander, M. Li, D.N. Bryant, L.L. Lindsey, N. Lurndahl, G.R. Rowden, R.L. Shoemaker, T.M. Wolf, P.A. Larsen, S.S. Lichtenberg); University of Minnesota, Minneapolis, Minnesota, USA (M. Li, P.R. Christenson, S.-H. Oh); Creighton University, Omaha, Nebraska, USA (J.C. Bartz)

Main Article

Figure 7

Results of real-time quaking-induced conversion assays conducted on meat grinder swab samples in study of detection and decontamination of chronic wasting disease prions during venison processing. Swab samples were taken after grinder parts were soaked in 1 of 4 decontaminants (after chronic wasting disease–positive muscle was passed through each): dish soap (top row), Dawn brand (Procter & Gamble, https://dawn-dish.com); Virkon-S (second row), 2% potassium peroxymonosulfate (Lanxess AG, https://lanxess.com); and bleach, 10% vol/vol (7,500 ppm) (third row) and 40% vol/vol (30,000 ppm) (bottom row) commercial bleach solutions (7.5% sodium hypochlorite; The Clorox Company, https://www.clorox.com). A) Rate of amyloid formation; B) maxpoint ratio (ratio of the maximum value to the initial reading); C) maximum slope. Each dot is an average of a single biological replicate (consisting of 8 technical replicates). PC, plate cutter; SRT, screw ring threads; WS, worm spindle.

Figure 7. Results of real-time quaking-induced conversion assays conducted on meat grinder swab samples in study of detection and decontamination of chronic wasting disease prions during venison processing. Swab samples were taken after grinder parts were soaked in 1 of 4 decontaminants (after chronic wasting disease–positive muscle was passed through each): dish soap (top row), Dawn brand (Procter & Gamble, https://dawn-dish.com); Virkon-S (second row), 2% potassium peroxymonosulfate (Lanxess AG, https://lanxess.com); and bleach, 10% vol/vol (7,500 ppm) (third row) and 40% vol/vol (30,000 ppm) (bottom row) commercial bleach solutions (7.5% sodium hypochlorite; The Clorox Company, https://www.clorox.com). A) Rate of amyloid formation; B) maxpoint ratio (ratio of the maximum value to the initial reading); C) maximum slope. Each dot is an average of a single biological replicate (consisting of 8 technical replicates). PC, plate cutter; SRT, screw ring threads; WS, worm spindle.

Main Article

Page created: February 07, 2025
Page updated: March 24, 2025
Page reviewed: March 24, 2025
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