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Volume 31, Number 4—April 2025
Research

Detection and Decontamination of Chronic Wasting Disease Prions during Venison Processing

Marissa Milstein, Sarah C. Gresch, Marc D. Schwabenlander, Manci Li, Jason C. Bartz, Damani N. Bryant, Peter R. Christenson, Laramie L. Lindsey, Nicole Lurndahl, Sang-Hyun Oh, Gage R. Rowden, Rachel L. Shoemaker, Tiffany M. Wolf, Peter A. Larsen, and Stuart S. LichtenbergComments to Author 
Author affiliation: University of Minnesota, St. Paul, Minnesota, USA (M. Milstein, S.C. Gresch, M.D. Schwabenlander, M. Li, D.N. Bryant, L.L. Lindsey, N. Lurndahl, G.R. Rowden, R.L. Shoemaker, T.M. Wolf, P.A. Larsen, S.S. Lichtenberg); University of Minnesota, Minneapolis, Minnesota, USA (M. Li, P.R. Christenson, S.-H. Oh); Creighton University, Omaha, Nebraska, USA (J.C. Bartz)

Main Article

Table 2

Results of testing of decontaminants in study of detection and decontamination of CWD prions during venison processing

Substance† Unspiked decontaminant control samples
Decontaminant control samples spiked with CWD-positive homogenate
Decontaminants rinsed then spiked with CWD-positive homogenate
No. positive p value
No. positive p value
No. positive p value
RAF MPR MS RAF MPR MS RAF MPR MS
Dish soap 0/3 1.0000 0.0048 0.5000 3/3 0.0029 0.0048 0.0048 3/3 0.0029 0.0048 0.0048
0.0047 0.0048 0.0857 0.0029 0.0048 0.0048 0.0029 0.0048 0.0070
1.0000 0.6191 0.7619 0.0029 0.0048 0.0048 0.0029 0.0048 0.0048
Briotech 0/3 0.5000 0.0857 0.0857 3/3 0.0029 0.0070 0.0070 3/3 0.0029 0.0048 0.0048
0.3593 0.1762 0.1762 0.0029 0.0070 0.0070 0.0029 0.0048 0.0048
0.9146 0.5429 0.5426 0.0025 0.0048 0.0048 0.0029 0.0048 0.0048
Virkon-S 0/3 0.5000 0.4571 0.3048 0.0126 0.0048 0.0048 3/3 0.0029 0.0048 0.0048
0.5000 0.0333 0.0571 1/3 0.0471 0.0571 0.1962 0.0029 0.0048 0.0048
0.8463 0.1286 0.1286 0.1537 0.1286 0.7619 0.0029 0.0048 0.0048
10% bleach 0/3 1.0000 0.8714 0.9429 0/3 0.1537 0.3810 0.5847 3/3 0.0029 0.0048 0.0048
1.0000 0.5429 0.9655 1.0000 0.3810 0.9879 0.0028 0.0070 0.0070
1.0000 0.0333 0.6191 1.0000 0.6191 0.9328 0.0029 0.0070 0.0070
40% bleach 0/3 0.5522 0.5429 0.8038 0/3 1.0000 0.0070 0.9963 3/3 0.0029 0.0070 0.0070
0.5522 0.6952 0.8714 1.0000 0.0070 0.9963 0.0029 0.0070 0.0070
0.5522 0.3048 0.7619 1.0000 0.0346 0.9789 0.0029 0.0070 0.0070

*Selected decontaminants were tested to determine if they directly interfered with the real-time quaking-induced conversion assay. CWD-positive homogenate was added to surfaces actively being treated, and after rinsing away the decontaminants to assess if they inhibited prion seeding activity. Bold indicates sample replicates found to have statistically significant seeding activity (p<0.05). CWD, chronic wasting disease; MPR, maxpoint ratio; MS, maximum slope; RAF, rate of amyloid formation. †Dish soap, Dawn brand (Procter & Gamble, https://dawn-dish.com); Briotech, 0.02% hypochlorous acid (https://briotechusa.shop); Virkon-S, 2% potassium peroxymonosulfate (Lanxess AG, https://lanxess.com); bleach, 10% vol/vol (7,500 ppm) and 40% vol/vol (30,000 ppm) commercial bleach solutions (7.5% sodium hypochlorite; The Clorox Company, https://www.clorox.com).

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Page created: February 07, 2025
Page updated: March 24, 2025
Page reviewed: March 24, 2025
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